ArticleBiophysical journal2016
The Affinity of Cholesterol for Different Phospholipids Affects Lateral Segregation in Bilayers.
Article in Biophysical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 74 citations in OpenAlex.
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- Cholesterol-dependent amyloid β production: space for multifarious interactions between amyloid precursor protein, secretases, and cholesterol.Cell & bioscience · 2023Review
- Brain Energy Metabolism: Astrocytes in Neurodegenerative Diseases.CNS neuroscience & therapeutics · 2023Review
- Role of polyunsaturated phospholipids in liquid-ordered and liquid-disordered phases.RSC advances · 2021Article
- Connecting the dots: from nanodomains to physiological functions of REMORINs.Plant physiology · 2021Review
- Coating of a Novel Antimicrobial Nanoparticle with a Macrophage Membrane for the Selective Entry into Infected Macrophages and Killing of Intracellular Staphylococci.Advanced functional materials · 2020Article
- Sphingomyelin Acyl Chains Influence the Formation of Sphingomyelin- and Cholesterol-Enriched Domains.Biophysical journal · 2020Article
- Spontaneous Membrane Nanodomain Formation in the Absence or Presence of the Neurotransmitter Serotonin.Frontiers in cell and developmental biology · 2020Article
- On the existence of endocytosis driven by membrane phase separations.Biochimica et biophysica acta. Biomembranes · 2020Review
- Impact of Acyl Chain Mismatch on the Formation and Properties of Sphingomyelin-Cholesterol Domains.Biophysical journal · 2019Article
- With Lipid Rafts, Context Is Everything.Biophysical journal · 2019Article
- Lateral Segregation of Palmitoyl Ceramide-1-Phosphate in Simple and Complex Bilayers.Biophysical journal · 2019Article
- Cholesterol-Dependent Bending Energy Is Important in Cholesterol Distribution of the Plasma Membrane.Biophysical journal · 2019Article
- Cholesterol Functionalization of Gold Nanoparticles Enhances Photoactivation of Neural Activity.ACS chemical neuroscience · 2019Article
- The Affinity of Sterols for Different Phospholipid Classes and Its Impact on Lateral Segregation.Biophysical journal · 2019Article
- Sphingomyelin Stereoisomers Reveal That Homophilic Interactions Cause Nanodomain Formation.Biophysical journal · 2018Article
- The Phase Behavior and Organization of Sphingomyelin/Cholesterol Membranes: A Deuterium NMR Study.Biophysical journal · 2018Article
- Food Ingredients That Inhibit Cholesterol Absorption.Preventive nutrition and food science · 2017Review
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Saturated and unsaturated phospholipids (PLs) can segregate into lateral domains. The preference of cholesterol for saturated acyl chains over monounsaturated, and especially polyunsaturated ones, may also affect lateral segregation. Here we have studied how cholesterol influenced the lateral segregation of saturated and unsaturated PLs, for which cholesterol had a varying degree of affinity. The fluorescence lifetime of trans-parinaric acid reported the formation of ordered domains (gel or liquid-ordered (lo)) in bilayers composed of different unsaturated phosphatidylcholines, and dipalmitoyl-phosphatidylcholine or n-palmitoyl-sphingomyelin, in the presence or absence of cholesterol. We observed that cholesterol facilitated lateral segregations and the degree of facilitation correlated with the relative affinity of cholesterol for the different PLs in the bilayers. Differential scanning calorimetry and (2)H nuclear magnetic resonance showed that cholesterol increased the thermostability of both the gel and lo-domains. Increased number of double bonds in the unsaturated PL increased the order in the lo-domains, likely by enriching the ordered domains in saturated lipids and cholesterol. This supported the conclusions from the trans-parinaric acid experiments, and offers insight into how cholesterol facilitated lateral segregation. In conclusion, the relative affinity of cholesterol for different PLs appears to be an important determinant for the formation of ordered domains. Our data suggests that knowledge of the affinity of cholesterol for the different PLs in a bilayer allows prediction of the degree to which the sterol promotes lo-domain formation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.