ArticleBiophysical journal2016
Quantitative Localization Microscopy Reveals a Novel Organization of a High-Copy Number Plasmid.
Article in Biophysical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed.
- ProFiT-SPEci-FISH: a novel approach for linking plasmids to hosts in complex microbial communities at the single-cell level.Microbiome · 2025Article
- Dynamic plasmid clustering in Bacteria: Influence of transcriptional activity and host cellular states.Biochemistry and biophysics reports · 2025Article
- Antechodynamics and Antechokinetics: Dynamics and Kinetics of Antibiotic Resistance Biomolecules.Biomolecules · 2025Review
- Preventing Multimer Formation in Commonly Used Synthetic Biology Plasmids.ACS synthetic biology · 2025Article
- DNA Segregation in Enterobacteria.EcoSal Plus · 2023Review
- Development of high-copy number plasmids in Pseudoalteromonas haloplanktis TAC125.Applied microbiology and biotechnology · 2023Article
- Single-molecule counting applied to the study of GPCR oligomerization.Biophysical journal · 2022Review
- Confinement anisotropy drives polar organization of two DNA molecules interacting in a nanoscale cavity.Nature communications · 2022Article
- Segregational Drift Constrains the Evolutionary Rate of Prokaryotic Plasmids.Molecular biology and evolution · 2021Article
- Single-cell measurement of plasmid copy number and promoter activity.Nature communications · 2021Article
- An expectation-maximization approach to quantifying protein stoichiometry with single-molecule imaging.Bioinformatics advances · 2021Article
- Effects of spatial heterogeneity on bacterial genetic circuits.PLoS computational biology · 2020Article
- Microampere Electric Current Causes Bacterial Membrane Damage and Two-Way Leakage in a Short Period of Time.Applied and environmental microbiology · 2020Article
- Precision design of stable genetic circuits carried in highly-insulated E. coli genomic landing pads.Molecular systems biology · 2020Article
- Silver Ions Caused Faster Diffusive Dynamics of Histone-Like Nucleoid-Structuring Proteins in Live Bacteria.Applied and environmental microbiology · 2020Article
- Emergence of plasmid stability under non-selective conditions maintains antibiotic resistance.Nature communications · 2019Article
- Plasmid Localization and Partition inEcoSal Plus · 2019Review
- High-Copy-Number Plasmid Segregation-Single-Molecule Dynamics in Single Cells.Biophysical journal · 2019Article
- Genetic control of ColE1 plasmid stability that is independent of plasmid copy number regulation.Current genetics · 2019Article
- SmallFrontiers in microbiology · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The maintenance of high-copy number plasmids within bacteria had been commonly thought to result from free diffusion and random segregation. Recent microscopy experiments, however, observed high-copy number plasmids clustering into discrete foci, which seemed to contradict this model, and hinted at an undiscovered active mechanism, as often found in low-copy number plasmids. We recently investigated the cellular organization of a ColE1-derivative plasmid in Escherichia coli bacteria using quantitative superresolved microscopy based on single-molecule localization in combination with single-molecule fluorescence in situ hybridization (smFISH). We observed that many of the plasmids aggregated into large clusters, although most of the plasmids were randomly distributed throughout the bacteria, minus an excluded volume about the chromosomal DNA. Our results indicate that neither of the previous models completely encompasses the behavior of high-copy number plasmids. We also found many plasmids within the chromosomal volume, providing further evidence that the nucleoid does not fully exclude DNA and RNA.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.