ArticleJournal of exercise nutrition & biochemistry2016
Characterization of the metabolic effect of β-alanine on markers of oxidative metabolism and mitochondrial biogenesis in skeletal muscle.
Article in Journal of exercise nutrition & biochemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 44 citations in OpenAlex.
- Effect of carnosine or beta-alanine supplementation therapy for prediabetes or type 2 diabetes mellitus: a systematic review and meta-analysis of randomized controlled trials.BMC endocrine disorders · 2025Pooled it
- Tissue-dependent enzymatic control of N-acetyl-β-alanine by PTER.The Journal of biological chemistry · 2026Article
- Blocking β-alanine synthesis triggers widespread perturbations of energy and lipid metabolism in the brain.Molecular metabolism · 2026Article
- The Metabolite Differences in Vascular Smooth Muscle Cells of Abdominal Aortic Aneurysm Revealed by Untargeted Metabolomics.Biomedicines · 2026Article
- Metabolic disruption in salmonids following co-exposure to road runoff contaminants 6PPD-Quinone and 9,10-anthraquinone.The Science of the total environment · 2026Article
- Analysis of metabolic rewiring in MDR1-overexpressing drug-resistant glioblastoma.Frontiers in pharmacology · 2026Article
- Metabolomic biomarkers predict long-term physical function in survivors of acute respiratory failure.Respiratory research · 2025Article
- Metabolomic Biomarkers Predict Long-term Physical Function in Survivors of Acute Respiratory Failure.Research square · 2025Article
- Guanidinoacetic Acid and Methionine Supplementation Improve the Growth Performance of Beef Cattle via Regulating the Antioxidant Levels and Protein and Lipid Metabolisms in Serum and Liver.Antioxidants (Basel, Switzerland) · 2025Article
- AARS2-catalyzed lactylation induces follicle development and premature ovarian insufficiency.Cell death discovery · 2025Article
- Integrated Metabolomic and Transcriptomic Analysis Revealed the Mechanism of BHPF Exposure in Endometrium.Toxics · 2025Article
- Metabolomics identifies plasma biomarkers of localized radiation injury.Scientific reports · 2025Article
- Comparative Impact of Organic Grass-Fed and Conventional Cattle-Feeding Systems on Beef and Human Postprandial Metabolomics-A Randomized Clinical Trial.Metabolites · 2024Article
- Reposition: Focalizing β-Alanine Metabolism and the Anti-Inflammatory Effects of Its Metabolite Based on Multi-Omics Datasets.International journal of molecular sciences · 2024Article
- Identification and validation of diagnostic biomarkers for intrahepatic cholestasis of pregnancy based on untargeted and targeted metabolomics analyses of urine metabolite profiles.BMC pregnancy and childbirth · 2023Article
- Role of the Mitochondrial Permeability Transition in Bone Metabolism and Aging.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2023Article
- Integrated Multilayer Omics Reveals the Genomic, Proteomic, and Metabolic Influences of Histidyl Dipeptides on the Heart.Journal of the American Heart Association · 2022Article
- Dynamic changes in the yak rumen eukaryotic community and metabolome characteristics in response to feed type.Frontiers in veterinary science · 2022Article
- Response of Bovine Cumulus-Oocytes Complexes to Energy Pathway Inhibition during In Vitro Maturation.Genes · 2021Article
- Effects of Dietary Supplements on Adaptations to Endurance Training.Sports medicine (Auckland, N.Z.) · 2020Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeβ-alanine is a common component of numerous sports supplements purported to improve athletic performance through enhanced carnosine biosynthesis and related intracellular buffering. To date, the effects of β-alanine on oxidative metabolism remain largely unexplored. This work investigated the effects of β-alanine on the expression of proteins which regulate cellular energetics.
methodsC2C12 myocytes were cultured and differentiated under standard conditions followed by treatment with either β-alanine or isonitrogenous non-metabolizable control D-alanine at 800μM for 24 hours. Metabolic gene and protein expression were quantified by qRT-PCR and immunoblotting, respectively. Glucose uptake and oxygen consumption were measured via fluorescence using commercially available kits.
resultsβ-alanine-treated myotubes displayed significantly elevated markers of improved oxidative metabolism including elevated peroxisome proliferator-activated receptor β/δ (PPARβ/δ) and mitochondrial transcription factor a (TFAM) which led to increased mitochondrial content (evidenced by concurrent increases in cytochrome c content). Additionally, β-alanine-treated cells exhibited significantly increased oxygen consumption compared to control in a PPARβ/δ-dependent manner. β-alanine significantly enhanced expression of myocyte enhancer factor 2 (MEF-2) leading to increased glucose transporter 4 (GLUT4) content.
conclusionβ-alanine appears to increase cellular oxygen consumption as well as the expression of several cellular proteins associated with improved oxidative metabolism, suggesting β-alanine supplementation may provide additional metabolic benefit (although these observations require in vivo experimental verification).
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