Evidence map›Paper›PMID 27508088›Full record

ArticleWestern Pacific surveillance and response journal : WPSAR

External quality assessment of dengue and chikungunya diagnostics in the Asia Pacific region, 2015.

Li Ting Soh, Raynal C Squires, Li Kiang Tan, Kwoon Yong Pok, HuiTing Yang, Christina Liew, Aparna Singh Shah, John Aaskov, Sazaly Abubakar, Futoshi Hasabe and 2 more

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Western Pacific surveillance and response journal : WPSAR. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
2.1field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Article
  3. A review of dengue diagnostics and implications for surveillance and control.Transactions of the Royal Society of Tropical Medicine and Hygiene · 2019
    Review
  4. Chikungunya Virus.Clinics in laboratory medicine · 2017
    Review
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 1 country.

Li Ting SohWHO Collaborating Centre for Reference and Research of Arbovirus and their Associated Vectors, Environmental Health Institute, National Environment Agency, Singapore.; Both authors contributed equally in the writing of this paper.
Raynal C SquiresEmerging Disease Surveillance and Response, Division of Health Security and Emergencies, World Health Organization Regional Office for the Western Pacific, Manila, Philippines.; Both authors contributed equally in the writing of this paper.
Li Kiang TanWHO Collaborating Centre for Reference and Research of Arbovirus and their Associated Vectors, Environmental Health Institute, National Environment Agency, Singapore .
Kwoon Yong PokWHO Collaborating Centre for Reference and Research of Arbovirus and their Associated Vectors, Environmental Health Institute, National Environment Agency, Singapore .
HuiTing YangWHO Collaborating Centre for Reference and Research of Arbovirus and their Associated Vectors, Environmental Health Institute, National Environment Agency, Singapore .
Christina LiewWHO Collaborating Centre for Reference and Research of Arbovirus and their Associated Vectors, Environmental Health Institute, National Environment Agency, Singapore .
Aparna Singh ShahBlood Safety and Laboratory Technology, Communicable Diseases Department, World Health Organization Regional Office for the South East-Asia, New Delhi, India .
John AaskovWHO Collaborating Centre for Reference and Research of Arbovirus and their Associated Vectors, Environmental Health Institute, National Environment Agency, Singapore .
Sazaly AbubakarWHO Collaborating Centre for Arbovirus Reference and Research (Dengue/Severe Dengue), Tropical Infectious Diseases Research and Education Centre, Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia .
Futoshi HasabeWHO Collaborating Centre for Reference and Research on Tropical and Emerging Virus Diseases, Nagasaki University, Nagasaki, Japan .
Lee Ching NgWHO Collaborating Centre for Reference and Research of Arbovirus and their Associated Vectors, Environmental Health Institute, National Environment Agency, Singapore .
Frank KoningsEmerging Disease Surveillance and Response, Division of Health Security and Emergencies, World Health Organization Regional Office for the Western Pacific, Manila, Philippines .
World Health Organization Regional Office for the Western Pacific · PH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo conduct an external quality assessment (EQA) of dengue and chikungunya diagnostics among national-level public health laboratories in the Asia Pacific region following the first round of EQA for dengue diagnostics in 2013.

methodsTwenty-four national-level public health laboratories performed routine diagnostic assays on a proficiency testing panel consisting of two modules. Module A contained serum samples spiked with cultured dengue virus (DENV) or chikungunya virus (CHIKV) for the detection of nucleic acid and DENV non-structural protein 1 (NS1) antigen. Module B contained human serum samples for the detection of anti-DENV antibodies.

resultsAmong 20 laboratories testing Module A, 17 (85%) correctly detected DENV RNA by reverse transcription polymerase chain reaction (RT-PCR), 18 (90%) correctly determined serotype and 19 (95%) correctly identified CHIKV by RT-PCR. Ten of 15 (66.7%) laboratories performing NS1 antigen assays obtained the correct results. In Module B, 18/23 (78.3%) and 20/20 (100%) of laboratories correctly detected anti-DENV IgM and IgG, respectively. Detection of acute/recent DENV infection by both molecular (RT-PCR) and serological methods (IgM) was available in 19/24 (79.2%) participating laboratories. DISCUSSION: Accurate laboratory testing is a critical component of dengue and chikungunya surveillance and control. This second round of EQA reveals good proficiency in molecular and serological diagnostics of these diseases in the Asia Pacific region. Further comprehensive diagnostic testing, including testing for Zika virus, should comprise future iterations of the EQA.

Indexed as

Outcome and Process Assessment, Health CareAntibodies, ViralAsiaChikungunya FeverChikungunya virusDengueDengue VirusHumansLaboratoriesAntibodies, Viral

Identifiers

PMID27508088
PMCPMC4957610
OpenAlexW2474529744

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.