ArticleFrontiers in microbiology2016
Antibiotic Susceptibility Testing of the Gram-Negative Bacteria Based on Flow Cytometry.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 48 citations in OpenAlex.
- Magnesium modulates phospholipid metabolism to promote bacterial phenotypic resistance to antibiotics.eLife · 2025Article
- Flow Cytometry as a Rapid and Valuable Method in Investigation of Colistin Resistance in Carbapenem-ResistantAntibiotics (Basel, Switzerland) · 2024Article
- Fluorescence Microscopy: Determination of Meropenem Activity againstAntibiotics (Basel, Switzerland) · 2023Article
- Current and Future Flow Cytometry Applications Contributing to Antimicrobial Resistance Control.Microorganisms · 2023Review
- Single-cell scattering and auto-fluorescence-based fast antibiotic susceptibility testing for gram-negative and gram-positive bacteria.Frontiers in microbiology · 2023Article
- Determination of antibiotic susceptibility of bacteria by flow cytometric method.World journal of microbiology & biotechnology · 2022Article
- Article
- Rapid high-resolution detection of colistin resistance in Gram-negative bacteria using flow cytometry: a comparison with broth microdilution, a commercial screening test and WGS.The Journal of antimicrobial chemotherapy · 2021Article
- The Autofluorescence Patterns ofPathogens (Basel, Switzerland) · 2021Article
- Rapid Determination of Antibiotic Resistance inFrontiers in microbiology · 2021Article
- Rapid Detection and Antibiotic Susceptibility of UropathogenicMicroorganisms · 2020Article
- Effect of subinhibitory exposure to quaternary ammonium compounds on the ciprofloxacin susceptibility of Escherichia coli strains in animal husbandry.BMC microbiology · 2020Article
- Rapid determination of carbapenem resistance by low-cost colorimetric methods: Propidium Iodide and alamar blue staining.Journal of microbiology (Seoul, Korea) · 2020Article
- 3D microfluidic gradient generator for combination antimicrobial susceptibility testing.Microsystems & nanoengineering · 2020Article
- Rapid antimicrobial susceptibility testing and β-lactam-induced cell morphology changes of Gram-negative biological threat pathogens by optical screening.BMC microbiology · 2018Article
- Direct-qPCR Assay for Coupled Identification and Antimicrobial Susceptibility Testing of Neisseria gonorrhoeae.ACS infectious diseases · 2018Article
- Strong increase in the autofluorescence of cells signals struggle for survival.Scientific reports · 2018Article
- Novel Antibiotic Testing Approaches Reveal Reduced Antibiotic Efficacy Against Shiga Toxin-ProducingFrontiers in microbiology · 2018Article
- A New Series of Pyrrole-Based Chalcones: Synthesis and Evaluation of Antimicrobial Activity, Cytotoxicity, and Genotoxicity.Molecules (Basel, Switzerland) · 2017Article
- Strain identification and quorum sensing inhibition characterization of marine-derivedRoyal Society open science · 2017Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapidly treating infections with adequate antibiotics is of major importance. This requires a fast and accurate determination of the antibiotic susceptibility of bacterial pathogens. The most frequently used methods are slow because they are based on the measurement of growth inhibition. Faster methods, such as PCR-based detection of determinants of antibiotic resistance, do not always provide relevant information on susceptibility, particularly that which is not genetically based. Consequently, new methods, such as the detection of changes in bacterial physiology caused by antibiotics using flow cytometry and fluorescent viability markers, are being explored. In this study, we assessed whether Alexa Fluor® 633 Hydrazide (AFH), which targets carbonyl groups, can be used for antibiotic susceptibility testing. Carbonylation of cellular macromolecules, which increases in antibiotic-treated cells, is a particularly appropriate to assess for this purpose because it is irreversible. We tested the susceptibility of clinical isolates of Gram-negative bacteria, Escherichia coli and Pseudomonas aeruginosa, to antibiotics from the three classes: β-lactams, aminoglycosides, and fluoroquinolones. In addition to AFH, we used TO-PRO®-3, which enters cells with damaged membranes and binds to DNA, and DiBAC4 (3), which enters cells with depolarized membranes. We also monitored antibiotic-induced morphological alterations of bacterial cells by analyzing light scattering signals. Although all tested dyes and light scattering signals allowed for the detection of antibiotic-sensitive cells, AFH proved to be the most suitable for the fast and reliable detection of antibiotic susceptibility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.