ArticleParasites & vectors2016
Isolation and genotyping of Acanthamoeba spp. from Acanthamoeba meningitis/ meningoencephalitis (AME) patients in India.
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16 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.
- The Implication of Horizontal Gene Transfer BetweenPathogens (Basel, Switzerland) · 2026Pooled it
- Beyond granulomatous amoebic encephalitis: changing epidemiology and an expanding spectrum of non-flagellated amoebic CNS infection in India.Infection · 2026Review
- Experimental Granulomatous Amebic Encephalitis Caused byTropical medicine and infectious disease · 2024Article
- Immunogens in Balamuthia mandrillaris: a proteomic exploration.Parasitology research · 2024Article
- Detection and Confirmation ofJournal of parasitology research · 2024Article
- The Epidemiology and Clinical Features of Non-KeratitisOpen forum infectious diseases · 2023Article
- Insights into genome evolution, pan-genome, and phylogenetic implication through mitochondrial genome sequence of Naegleria fowleri species.Scientific reports · 2022Article
- Direct Metagenomic Diagnosis of Community-Acquired Meningitis: State of the Art.Frontiers in microbiology · 2022Review
- Various brain-eating amoebae: the protozoa, the pathogenesis, and the disease.Frontiers of medicine · 2021Review
- Aptamer-based approaches for the detection of waterborne pathogens.International microbiology : the official journal of the Spanish Society for Microbiology · 2021Review
- Identification of T3 and T4 Genotypes of Acanthamoeba sp. in Dust Samples Isolated from Air Conditioning Equipment of Public Hospital of Ituiutaba-MG.Current microbiology · 2020Article
- Genotyping ofThe Indian journal of medical research · 2018Article
- Article
- Something Amiss: Acanthamoeba Meningoencephalitis: Report of Two Cases from Kolkata, India.Annals of Indian Academy of NeurologyArticle
- Free-living amoebic encephalitis - Case series.Tropical parasitologyArticle
- Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAcanthamoeba spp. are free-living ubiquitous protozoans capable of causing Acanthamoeba meningitis/meningoencephalitis (AME) of the central nervous system in humans. Acanthamoeba spp. are divided into 20 different genotypes (T1-T20) on the basis of variation in nucleotide sequences of the 18S rRNA gene. The objective of this study was to identify the genotypes of Acanthamoeba spp. in patients of Acanthamoeba meningitis/meningoencephalitis (AME) using 18S rRNA gene-based PCR assay. The present study provides information regarding the involvement of the most prevalent and predominant genotype of Acanthamoeba spp. in Acanthamoeba meningitis/meningoencephalitis infections in India.
methodsCerebrospinal fluid (CSF) was collected from 149 clinically suspected Acanthamoeba meningitis/meningoencephalitis (AME) patients reporting to the outpatient department/causality services of the Neurosciences Centre, AIIMS, New Delhi, India during the past five years. Samples were inoculated onto 2 % non-nutrient agar plates overlaid with E. coli and incubated at 30 °C for 14 days. Among 149 suspected patients, ten were found culture-positive for Acanthamoeba spp. out of which six isolates were established in axenic culture for molecular analysis. DNA was isolated and a PCR assay was performed for amplification of the Diagnostic fragment 3 (DF3) (~280 bp) region of the 18S rRNA gene from axenic culture of six Acanthamoeba spp. isolates. Rns genotyping was performed on the basis of the variation in nucleotide sequences of DF3 region of the 18S rRNA gene.
resultsIn the phylogenetic analysis, all of the six Acanthamoeba spp. isolates were found to belong to genotype T4. The sequence homology search for these six isolates in the NCBI databank showed homology with the available strains of Acanthamoeba spp. The newly generated sequences are available in the GenBank database under accession numbers KT004416-KT004421.
conclusionsIn the present study, genotype T4 was found as the most prevalent and predominant genotype in Acanthamoeba meningitis/ meningoencephalitis infections. Hence further studies are needed to develop optimal therapeutic strategy against Acanthamoeba spp. of genotype T4 to combat against the infections.
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