ArticleBMC genomics2016
Design and application of a target capture sequencing of exons and conserved non-coding sequences for the rat.
Article in BMC genomics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Detection of Genetic Variations in Children with Tetralogy of Fallot Using Whole Exome Sequencing Technology Integrated Bioinformatics Analysis.Genetic testing and molecular biomarkers · 2024Article
- Whole exome and transcript profiling of liver following aflatoxin B1 exposure in rats.Journal of applied toxicology : JAT · 2023Article
- A domestic cat whole exome sequencing resource for trait discovery.Scientific reports · 2021Article
- Comparative genomic analysis of inbred rat strains reveals the existence of ancestral polymorphisms.Mammalian genome : official journal of the International Mammalian Genome Society · 2020Article
- Long-read sequence capture of the haemoglobin gene clusters across codfish species.Molecular ecology resources · 2019Article
- Whole exome sequencing in the rat.BMC genomics · 2018Article
- Article
- A deletion in the intergenic region upstream of Ednrb causes head spot in the rat strain KFRS4/Kyo.BMC genetics · 2017Article
- Application of target capture sequencing of exons and conserved non-coding sequences to 20 inbred rat strains.Genomics data · 2016Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTarget capture sequencing is an efficient approach to directly identify the causative mutations of genetic disorders. To apply this strategy to laboratory rats exhibiting various phenotypes, we developed a novel target capture probe set, TargetEC (target capture for exons and conserved non-coding sequences), which can identify mutations not only in exonic regions but also in conserved non-coding sequences and thus can detect regulatory mutations.
resultsTargetEC covers 1,078,129 regions spanning 146.8 Mb of the genome. We applied TargetEC to four inbred rat strains (WTC/Kyo, WTC-swh/Kyo, PVG/Seac, and KFRS4/Kyo) maintained by the National BioResource Project for the Rat in Japan, and successfully identified mutations associated with these phenotypes, including one mutation detected in a conserved non-coding sequence.
conclusionsThe method developed in this study can be used to efficiently identify regulatory mutations, which cannot be detected using conventional exome sequencing, and will help to deepen our understanding of the relationships between regulatory mutations and associated phenotypes.
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