ArticlePlant physiology2016
d-Lactate Dehydrogenase Links Methylglyoxal Degradation and Electron Transport through Cytochrome c.
Open access · bronzeAbstract read
In one paragraph
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
4.7field-weighted citation impact, top 5% of its field
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 59 citations in OpenAlex.
- Natural variation in Arabidopsis thaliana highlights a key role of glyoxalase I;2 in detoxifying glucose-derived reactive carbonyl species.BMC plant biology · 2025Article
- Characterization of a Periplasmic D-Malate:CytochromeInternational journal of molecular sciences · 2025Article
- Induced Resistance against Pseudomonas syringae pv. lachrymans in Cucumber by Spraying Cell-Free Microalgae Supernatant.The plant pathology journal · 2025Article
- Cloning and Functional Analysis of Glyoxalase I GeneInternational journal of molecular sciences · 2025Article
- Lactate metabolic reprogramming and histone lactylation modification in sepsis.International journal of biological sciences · 2025Review
- Decrypting Molecular Mechanisms Involved in Counteracting Copper and Nickel Toxicity in Jack Pine (Plants (Basel, Switzerland) · 2024Article
- A translation proofreader of archaeal origin imparts multi-aldehyde stress tolerance to land plants.eLife · 2024Article
- Viridiplantae-specific GLXI and GLXII isoforms co-evolved and detoxify glucosone in planta.Plant physiology · 2023Article
- Glyoxalase I activity affects Arabidopsis sensitivity to ammonium nutrition.Plant cell reports · 2022Article
- A proteomics approach to decipher a sticky CHO situation.Biotechnology and bioengineering · 2022Article
- Color recycling: metabolization of apocarotenoid degradation products suggests carbon regeneration via primary metabolic pathways.Plant cell reports · 2022Article
- Reductive stress triggers ANAC017-mediated retrograde signaling to safeguard the endoplasmic reticulum by boosting mitochondrial respiratory capacity.The Plant cell · 2022Article
- The Roles of Coenzyme Q in Disease: Direct and Indirect Involvement in Cellular Functions.International journal of molecular sciences · 2021Review
- Origin and Function of Structural Diversity in the Plant Specialized Metabolome.Plants (Basel, Switzerland) · 2021Review
- Glutathione Metabolism in Plants under Stress: Beyond Reactive Oxygen Species Detoxification.Metabolites · 2021Review
- Human d-lactate dehydrogenase deficiency byJIMD reports · 2021Article
- Mitochondrial redox systems as central hubs in plant metabolism and signaling.Plant physiology · 2021Review
- CytochromePlants (Basel, Switzerland) · 2021Article
- Algae as New Kids in the Beneficial Plant Microbiome.Frontiers in plant science · 2021Review
- A D-lactate dehydrogenase from rice is involved in conferring tolerance to multiple abiotic stresses by maintaining cellular homeostasis.Scientific reports · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 6 institutions in 2 countries.
Elina WelchenInstituto de Agrobiotecnología del Litoral, Consejo Nacional de Investigaciones Científicas y Técnicas, Cátedra de Biología Celular y Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 3000 Santa Fe, Argentina (E.W., L.G., D.H.G.);Institute of Developmental and Molecular Biology of Plants, Plant Molecular Physiology and Biotechnology Group, Heinrich-Heine-Universität, and Cluster of Excellence on Plant Sciences, 40225 Duesseldorf, Germany (J.S., J.W., V.G.M.);Plant Energy Biology Laboratory, Institute of Crop Science and Resource Conservation, Rheinische Friedrich-Wilhelms-Universität Bonn, 53113 Bonn, Germany (P.F., S.W., M.S.); andPflanzengenetik, Abteilung Pflanzenproteomik, Leibniz Universität Hannover, 30419 Hannover, Germany (P.S., H.-P.B.).ORCID 0000-0003-4025-573X
Jessica SchmitzInstituto de Agrobiotecnología del Litoral, Consejo Nacional de Investigaciones Científicas y Técnicas, Cátedra de Biología Celular y Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 3000 Santa Fe, Argentina (E.W., L.G., D.H.G.);Institute of Developmental and Molecular Biology of Plants, Plant Molecular Physiology and Biotechnology Group, Heinrich-Heine-Universität, and Cluster of Excellence on Plant Sciences, 40225 Duesseldorf, Germany (J.S., J.W., V.G.M.);Plant Energy Biology Laboratory, Institute of Crop Science and Resource Conservation, Rheinische Friedrich-Wilhelms-Universität Bonn, 53113 Bonn, Germany (P.F., S.W., M.S.); andPflanzengenetik, Abteilung Pflanzenproteomik, Leibniz Universität Hannover, 30419 Hannover, Germany (P.S., H.-P.B.).
Philippe FuchsInstituto de Agrobiotecnología del Litoral, Consejo Nacional de Investigaciones Científicas y Técnicas, Cátedra de Biología Celular y Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 3000 Santa Fe, Argentina (E.W., L.G., D.H.G.);Institute of Developmental and Molecular Biology of Plants, Plant Molecular Physiology and Biotechnology Group, Heinrich-Heine-Universität, and Cluster of Excellence on Plant Sciences, 40225 Duesseldorf, Germany (J.S., J.W., V.G.M.);Plant Energy Biology Laboratory, Institute of Crop Science and Resource Conservation, Rheinische Friedrich-Wilhelms-Universität Bonn, 53113 Bonn, Germany (P.F., S.W., M.S.); andPflanzengenetik, Abteilung Pflanzenproteomik, Leibniz Universität Hannover, 30419 Hannover, Germany (P.S., H.-P.B.).ORCID 0000-0001-6379-853X
Lucila GarcíaInstituto de Agrobiotecnología del Litoral, Consejo Nacional de Investigaciones Científicas y Técnicas, Cátedra de Biología Celular y Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 3000 Santa Fe, Argentina (E.W., L.G., D.H.G.);Institute of Developmental and Molecular Biology of Plants, Plant Molecular Physiology and Biotechnology Group, Heinrich-Heine-Universität, and Cluster of Excellence on Plant Sciences, 40225 Duesseldorf, Germany (J.S., J.W., V.G.M.);Plant Energy Biology Laboratory, Institute of Crop Science and Resource Conservation, Rheinische Friedrich-Wilhelms-Universität Bonn, 53113 Bonn, Germany (P.F., S.W., M.S.); andPflanzengenetik, Abteilung Pflanzenproteomik, Leibniz Universität Hannover, 30419 Hannover, Germany (P.S., H.-P.B.).ORCID 0000-0002-5109-2043
Stephan WagnerInstituto de Agrobiotecnología del Litoral, Consejo Nacional de Investigaciones Científicas y Técnicas, Cátedra de Biología Celular y Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 3000 Santa Fe, Argentina (E.W., L.G., D.H.G.);Institute of Developmental and Molecular Biology of Plants, Plant Molecular Physiology and Biotechnology Group, Heinrich-Heine-Universität, and Cluster of Excellence on Plant Sciences, 40225 Duesseldorf, Germany (J.S., J.W., V.G.M.);Plant Energy Biology Laboratory, Institute of Crop Science and Resource Conservation, Rheinische Friedrich-Wilhelms-Universität Bonn, 53113 Bonn, Germany (P.F., S.W., M.S.); andPflanzengenetik, Abteilung Pflanzenproteomik, Leibniz Universität Hannover, 30419 Hannover, Germany (P.S., H.-P.B.).ORCID 0000-0001-5369-7911
Judith WienstroerInstituto de Agrobiotecnología del Litoral, Consejo Nacional de Investigaciones Científicas y Técnicas, Cátedra de Biología Celular y Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 3000 Santa Fe, Argentina (E.W., L.G., D.H.G.);Institute of Developmental and Molecular Biology of Plants, Plant Molecular Physiology and Biotechnology Group, Heinrich-Heine-Universität, and Cluster of Excellence on Plant Sciences, 40225 Duesseldorf, Germany (J.S., J.W., V.G.M.);Plant Energy Biology Laboratory, Institute of Crop Science and Resource Conservation, Rheinische Friedrich-Wilhelms-Universität Bonn, 53113 Bonn, Germany (P.F., S.W., M.S.); andPflanzengenetik, Abteilung Pflanzenproteomik, Leibniz Universität Hannover, 30419 Hannover, Germany (P.S., H.-P.B.).
Peter SchertlInstituto de Agrobiotecnología del Litoral, Consejo Nacional de Investigaciones Científicas y Técnicas, Cátedra de Biología Celular y Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 3000 Santa Fe, Argentina (E.W., L.G., D.H.G.);Institute of Developmental and Molecular Biology of Plants, Plant Molecular Physiology and Biotechnology Group, Heinrich-Heine-Universität, and Cluster of Excellence on Plant Sciences, 40225 Duesseldorf, Germany (J.S., J.W., V.G.M.);Plant Energy Biology Laboratory, Institute of Crop Science and Resource Conservation, Rheinische Friedrich-Wilhelms-Universität Bonn, 53113 Bonn, Germany (P.F., S.W., M.S.); andPflanzengenetik, Abteilung Pflanzenproteomik, Leibniz Universität Hannover, 30419 Hannover, Germany (P.S., H.-P.B.).
Hans-Peter BraunInstituto de Agrobiotecnología del Litoral, Consejo Nacional de Investigaciones Científicas y Técnicas, Cátedra de Biología Celular y Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 3000 Santa Fe, Argentina (E.W., L.G., D.H.G.);Institute of Developmental and Molecular Biology of Plants, Plant Molecular Physiology and Biotechnology Group, Heinrich-Heine-Universität, and Cluster of Excellence on Plant Sciences, 40225 Duesseldorf, Germany (J.S., J.W., V.G.M.);Plant Energy Biology Laboratory, Institute of Crop Science and Resource Conservation, Rheinische Friedrich-Wilhelms-Universität Bonn, 53113 Bonn, Germany (P.F., S.W., M.S.); andPflanzengenetik, Abteilung Pflanzenproteomik, Leibniz Universität Hannover, 30419 Hannover, Germany (P.S., H.-P.B.).ORCID 0000-0002-4459-9727
Markus SchwarzländerInstituto de Agrobiotecnología del Litoral, Consejo Nacional de Investigaciones Científicas y Técnicas, Cátedra de Biología Celular y Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 3000 Santa Fe, Argentina (E.W., L.G., D.H.G.);Institute of Developmental and Molecular Biology of Plants, Plant Molecular Physiology and Biotechnology Group, Heinrich-Heine-Universität, and Cluster of Excellence on Plant Sciences, 40225 Duesseldorf, Germany (J.S., J.W., V.G.M.);Plant Energy Biology Laboratory, Institute of Crop Science and Resource Conservation, Rheinische Friedrich-Wilhelms-Universität Bonn, 53113 Bonn, Germany (P.F., S.W., M.S.); andPflanzengenetik, Abteilung Pflanzenproteomik, Leibniz Universität Hannover, 30419 Hannover, Germany (P.S., H.-P.B.).ORCID 0000-0003-0796-8308
Daniel H GonzalezInstituto de Agrobiotecnología del Litoral, Consejo Nacional de Investigaciones Científicas y Técnicas, Cátedra de Biología Celular y Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 3000 Santa Fe, Argentina (E.W., L.G., D.H.G.);Institute of Developmental and Molecular Biology of Plants, Plant Molecular Physiology and Biotechnology Group, Heinrich-Heine-Universität, and Cluster of Excellence on Plant Sciences, 40225 Duesseldorf, Germany (J.S., J.W., V.G.M.);Plant Energy Biology Laboratory, Institute of Crop Science and Resource Conservation, Rheinische Friedrich-Wilhelms-Universität Bonn, 53113 Bonn, Germany (P.F., S.W., M.S.); andPflanzengenetik, Abteilung Pflanzenproteomik, Leibniz Universität Hannover, 30419 Hannover, Germany (P.S., H.-P.B.).
Veronica G MaurinoInstituto de Agrobiotecnología del Litoral, Consejo Nacional de Investigaciones Científicas y Técnicas, Cátedra de Biología Celular y Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 3000 Santa Fe, Argentina (E.W., L.G., D.H.G.);Institute of Developmental and Molecular Biology of Plants, Plant Molecular Physiology and Biotechnology Group, Heinrich-Heine-Universität, and Cluster of Excellence on Plant Sciences, 40225 Duesseldorf, Germany (J.S., J.W., V.G.M.);Plant Energy Biology Laboratory, Institute of Crop Science and Resource Conservation, Rheinische Friedrich-Wilhelms-Universität Bonn, 53113 Bonn, Germany (P.F., S.W., M.S.); andPflanzengenetik, Abteilung Pflanzenproteomik, Leibniz Universität Hannover, 30419 Hannover, Germany (P.S., H.-P.B.) veronica.maurino@uni-duesseldorf.de.ORCID 0000-0002-3124-1451
University of Bonn · DEConsejo Nacional de Investigaciones Científicas y Técnicas · ARHeinrich Heine University Düsseldorf · DELeibniz University Hannover · DECluster of Excellence on Plant Sciences · DEInstituto de Agrobiotecnología del Litoral · AR
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glycolysis generates methylglyoxal (MGO) as an unavoidable, cytotoxic by-product in plant cells. MGO scavenging is performed by the glyoxalase system, which produces d-lactate as an end product. d-Lactate dehydrogenase (d-LDH) is encoded by a single gene in Arabidopsis (Arabidopsis thaliana; At5g06580). It catalyzes in vitro the oxidation of d-lactate to pyruvate using flavin adenine dinucleotide as a cofactor; knowledge of its function in the context of the plant cell remains sketchy. Blue native-polyacrylamide gel electrophoresis of mitochondrial extracts combined with in gel activity assays using different substrates and tandem mass spectrometry allowed us to definitely show that d-LDH acts specifically on d-lactate, is active as a dimer, and does not associate with respiratory supercomplexes of the inner mitochondrial membrane. The combined use of cytochrome c (CYTc) loss-of-function mutants and respiratory complex III inhibitors showed that CYTc acts as the in vivo electron acceptor of d-LDH. CYTc loss-of-function mutants, as well as the d-LDH mutants, were more sensitive to d-lactate and MGO, indicating that they function in the same pathway. In addition, overexpression of d-LDH and CYTc increased tolerance to d-lactate and MGO Together with fine-localization of d-LDH, the functional interaction with CYTc in vivo strongly suggests that d-lactate oxidation takes place in the mitochondrial intermembrane space, delivering electrons to the respiratory chain through CYTc These results provide a comprehensive picture of the organization and function of d-LDH in the plant cell and exemplify how the plant mitochondrial respiratory chain can act as a multifunctional electron sink for reductant from cytosolic pathways.
Indexed as
ArabidopsisArabidopsis ProteinsBiocatalysisBlotting, WesternCells, CulturedCytochromes cElectron TransportLactic AcidL-Lactate DehydrogenaseMass SpectrometryMicroscopy, ConfocalMitochondrial MembranesMutationOxidation-ReductionOxygen ConsumptionPlants, Genetically ModifiedArabidopsis ProteinsCytochromes cLactic AcidL-Lactate DehydrogenasePyruvaldehydePyruvic Acid
Identifiers
What OpenQuestion holds
Textmetadata
Read underepoch 390
Registered trials
None linked
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.