ArticlePlant physiology2016
The DEK1 Calpain Linker Functions in Three-Dimensional Body Patterning in Physcomitrella patens.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- Calpains as Potential Regulators of Microtubule-Associated Cytokinesis: A Cross-Eukaryotic Unifying Hypothesis.Life (Basel, Switzerland) · 2026Article
- Computational optimization of DEK1 calpain domain solubility through integrated structural modelling and data-driven targeted mutagenesis.Scientific reports · 2026Article
- A FLOE-related protein regulates the two-dimensional to three-dimensional growth transition in the moss Physcomitrium patens.Development (Cambridge, England) · 2025Article
- CLE peptides act via the receptor-like kinase CRINKLY 4 inPlant signaling & behavior · 2024Article
- Regulation of developmental gatekeeping and cell fate transition by the calpain protease DEK1 in Physcomitrium patens.Communications biology · 2024Article
- Improved prime editing allows for routine predictable gene editing in Physcomitrium patens.Journal of experimental botany · 2023Article
- Article
- Genome-wide alternative polyadenylation dynamics underlying plant growth retardant-induced dwarfing of pomegranate.Frontiers in plant science · 2023Article
- Membrane-anchored calpains - hidden regulators of growth and development beyond plants?Frontiers in plant science · 2023Article
- Unravelling 3D growth in the moss Physcomitrium patens.Essays in biochemistry · 2022Article
- The bryophytes Physcomitrium patens and Marchantia polymorpha as model systems for studying evolutionary cell and developmental biology in plants.The Plant cell · 2022Review
- PpGRAS12 acts as a positive regulator of meristem formation in Physcomitrium patens.Plant molecular biology · 2021Article
- Review
- Challenges ofPlant signaling & behavior · 2020Article
- Genetic Regulation of the 2D to 3D Growth Transition in the Moss Physcomitrella patens.Current biology : CB · 2018Article
- An intragenic mutagenesis strategy in Physcomitrella patens to preserve intron splicing.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The DEFECTIVE KERNEL1 (DEK1) calpain is a conserved 240-kD key regulator of three-dimensional body patterning in land plants acting via mitotic cell plane positioning. The activity of the cytosolic C-terminal calpain protease is regulated by the membrane-anchored DEK1 MEM, which is connected to the calpain via the 600-amino acid residue Linker. Similar to the calpain and MEM domains, the Linker is highly conserved in the land plant lineage, the similarity dropping sharply compared with orthologous charophyte sequences. Using site-directed mutagenesis, we studied the effect on Physcomitrella patens development by deleting the Linker and two conserved Linker motifs. The results show that removal of the Linker has nearly the same effect as removal of the entire DEK1 gene. In contrast, deletion of the conserved Laminin_G3 (LG3) domain had a milder effect, perturbing leafy gametophore patterning and archegonia development. The LG3 domain from Marchantia polymorpha is fully functional in P. patens, whereas angiosperm sequences are not functional. Deletion of a C-terminal Linker subsegment containing a potential calpain autolytic site severely disturbs gametophore development. Finally, changing one of the three calpain active-site amino acid residues results in the same phenotype as deleting the entire DEK1 gene. Based on the conserved nature of animal and DEK1 calpains, we propose that the DEK1 MEM-Linker complex inactivates the calpain by forcing apart the two calpain subunits carrying the three amino acids of the active site.
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