ArticlePlant physiology2016
Effects of Auxins on PIN-FORMED2 (PIN2) Dynamics Are Not Mediated by Inhibiting PIN2 Endocytosis.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 51 citations in OpenAlex.
- Both brefeldin A and latrunculin B treatment induces cytoplasmic aggregation of plasma membrane-associated proteins in epidermal cells, but not in columella cells.Plant signaling & behavior · 2026Article
- Alterations in the proteomic profiles of Arabidopsis roots triggered by sodium chloride stress are exacerbated by impaired function of SYNAPTOTAGMIN 1.BMC plant biology · 2025Article
- Biosynthesis of very Long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development.The Plant journal : for cell and molecular biology · 2025Article
- Callus culture-derived regeneration and molecular characterization of regeneratedFrontiers in plant science · 2025Article
- Ubiquitin E3 ligases in the plant Arg/N-degron pathway.The Biochemical journal · 2024Review
- Over 25 years of decrypting PIN-mediated plant development.Nature communications · 2024Review
- Auxin-Mediated Lateral Root Development in Root Galls of Cucumber underPlants (Basel, Switzerland) · 2024Article
- SYNAPTOTAGMIN 4 is expressed mainly in the phloem and participates in abiotic stress tolerance in Arabidopsis.Frontiers in plant science · 2024Article
- Regulation of PIN-FORMED Protein Degradation.International journal of molecular sciences · 2023Review
- Specific expression ofPlant signaling & behavior · 2021Article
- No Time for Transcription-Rapid Auxin Responses in Plants.Cold Spring Harbor perspectives in biology · 2021Review
- What remains of the evidence for auxin feedback on PIN polarity patterns?Plant physiology · 2021Article
- Systematic analysis of specific and nonspecific auxin effects on endocytosis and trafficking.Plant physiology · 2021Article
- Auxin does not inhibit endocytosis of PIN1 and PIN2 auxin efflux carriers.Plant physiology · 2021Article
- Abscisic Acid Regulates the Root Growth Trajectory by Reducing Auxin TransporterFrontiers in plant science · 2021Article
- All Roads Lead to Auxin: Post-translational Regulation of Auxin Transport by Multiple Hormonal Pathways.Plant communications · 2020Review
- Auxin-induced actin cytoskeleton rearrangements require AUX1.The New phytologist · 2020Article
- Endosidin 2 accelerates PIN2 endocytosis and disturbs intracellular trafficking of PIN2, PIN3, and PIN4 but not of SYT1.PloS one · 2020Article
- A Conditional Mutation inFrontiers in plant science · 2020Article
- CsBRC1 inhibits axillary bud outgrowth by directly repressing the auxin efflux carrierProceedings of the National Academy of Sciences of the United States of America · 2019Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
By using the photoconvertible fluorescence protein Dendra2 as a tag we demonstrated that neither the naturally occurring auxins indole-3-acetic acid and indole-3-butyric acid, nor the synthetic auxin analogs 1-naphthaleneacetic acid and 2,4-dichlorophenoxyacetic acid nor compounds inhibiting polar auxin transport such as 2,3,5-triiodobenzoic acid and 1-N-naphthylphthalamic acid, were able to inhibit endocytosis of the putative auxin transporter PIN-FORMED2 (PIN2) in Arabidopsis (Arabidopsis thaliana) root epidermis cells. All compounds, except Indole-3-butyric acid, repressed the recovery of the PIN2-Dendra2 plasma membrane pool after photoconversion when they were used in high concentrations. The synthetic auxin analogs 1-naphthaleneacetic acid and 2,4-dichlorophenoxyacetic acid showed the strongest inhibition. Auxins and auxin transport inhibitors suppressed also the accumulation of both newly synthesized and endocytotic PIN2 pools in Brefeldin A compartments (BFACs). Furthermore, we demonstrated that all compounds are also interfering with BFAC formation. The synthetic auxin analogs caused the highest reduction in the number and size of BFACs. We concluded that auxins and inhibitors of auxin transport do affect PIN2 turnover in the cells, but it is through the synthetic rather than the endocytotic pathway. The study also confirmed inappropriateness of the BFA-based approach to study PIN2 endocytosis because the majority of PIN2 accumulating in BFACs is newly synthesized and not derived from the plasma membrane.
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