Evidence map›Paper›PMID 27505892›Full record

ArticleJournal of cell science2016

Visualizing red blood cell sickling and the effects of inhibition of sphingosine kinase 1 using soft X-ray tomography.

Michele C Darrow, Yujin Zhang, Bertrand P Cinquin, Elizabeth A Smith, Rosanne Boudreau, Ryan H Rochat, Michael F Schmid, Yang Xia, Carolyn A Larabell, Wah Chiu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cell science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Review
  8. KJournal of hematology & oncology · 2019
    Review
  9. Imaging cell morphology and physiology using X-rays.Biochemical Society transactions · 2019
    Review
  10. Article
  11. Article
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Michele C DarrowNational Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA.
Yujin ZhangDepartment of Biochemistry and Molecular Biology, University of Texas Health Science Center at Houston, Houston, TX 77030, USA.
Bertrand P CinquinDepartment of Anatomy, University of California San Francisco, San Francisco, CA 94143, USA Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Elizabeth A SmithDepartment of Anatomy, University of California San Francisco, San Francisco, CA 94143, USA Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Rosanne BoudreauDepartment of Anatomy, University of California San Francisco, San Francisco, CA 94143, USA Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Ryan H RochatNational Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA.
Michael F SchmidNational Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA.
Yang XiaDepartment of Biochemistry and Molecular Biology, University of Texas Health Science Center at Houston, Houston, TX 77030, USA University of Texas at Houston Graduate School of Biomedical Sciences, Houston, TX 77030, USA Department of Nephrology, The First Xiangya Hospital, Central South University, Changsha, Hunan 410008, People's Republic of China.
Carolyn A LarabellDepartment of Anatomy, University of California San Francisco, San Francisco, CA 94143, USA Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA wah@bcm.edu carolyn.larabell@ucsf.edu.
Wah ChiuNational Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA wah@bcm.edu carolyn.larabell@ucsf.edu.ORCID 0000-0002-8910-3078

Funding

Center for Protein Folding Machinery (RMI)PN2EY016525 · NEI · BAYLOR COLLEGE OF MEDICINE · PI CHIU, WAH · 2005 to 2015
$33.6M
3 D ELECTRON MICROSCOPY OF MACROMOLECULES (5P41RR002250-27)P41GM103832 · NIGMS · STANFORD UNIVERSITY · PI CHIU, WAH · 2012 to 2019
$15.1M
Trangenic Mouse Breeding CoreP01HL114457 · NHLBI · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI ZHANG, YUJIN · 2013 to 2017
$10.0M
TrainingP41GM103445 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LARABELL, CAROLYN A · 2012 to 2019
$8.2M
Metabolites, Sickle Cell Disease, and Novel TherapeuticsR01HL113574 · NHLBI · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI XIA, YANG · 2013 to 2016
$1.5M
NEI NIH HHS PN2 EY016525NHLBI NIH HHS P01 HL114457NHLBI NIH HHS R01 HL113574NIGMS NIH HHS P41 GM103445NIGMS NIH HHS P41 GM103832
6 · The paper itself

Abstract

Sickle cell disease is a destructive genetic disorder characterized by the formation of fibrils of deoxygenated hemoglobin, leading to the red blood cell (RBC) morphology changes that underlie the clinical manifestations of this disease. Using cryogenic soft X-ray tomography (SXT), we characterized the morphology of sickled RBCs in terms of volume and the number of protrusions per cell. We were able to identify statistically a relationship between the number of protrusions and the volume of the cell, which is known to correlate to the severity of sickling. This structural polymorphism allows for the classification of the stages of the sickling process. Recent studies have shown that elevated sphingosine kinase 1 (Sphk1)-mediated sphingosine 1-phosphate production contributes to sickling. Here, we further demonstrate that compound 5C, an inhibitor of Sphk1, has anti-sickling properties. Additionally, the variation in cellular morphology upon treatment suggests that this drug acts to delay the sickling process. SXT is an effective tool that can be used to identify the morphology of the sickling process and assess the effectiveness of potential therapeutics.

Indexed as

Anemia, Sickle CellAnimalsCell Surface ExtensionsErythrocytesHumansMicePhosphotransferases (Alcohol Group Acceptor)Protein Kinase InhibitorsSphingosine KinaseTomography, X-RayPhosphotransferases (Alcohol Group Acceptor)Protein Kinase InhibitorsSphingosine KinaseCryogenic soft X-ray tomographyRed blood cellRed cell morphologySickle cell diseaseSphingosine kinase inhibitor

Identifiers

PMID27505892
PMCPMC5047677

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.