ArticlePloS one2016
Developmental Toxicity of Zinc Oxide Nanoparticles to Zebrafish (Danio rerio): A Transcriptomic Analysis.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 132 citations in OpenAlex.
- Common Gene Expression Patterns in Environmental Model Organisms Exposed to Engineered Nanomaterials: A Meta-Analysis.Environmental science & technology · 2020Pooled it
- Nanotechnology-Based Immunostimulants in Aquaculture: Mechanisms, Advances and Practical Applications.Veterinary medicine and science · 2026Review
- Mechanistic paradigms of teratogenicity induced by metallic nanoparticles-a mini review.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2026Review
- Anxiety of microbially synthesized FeApplied microbiology and biotechnology · 2025Article
- Toxicity evaluation of zinc oxide nanoparticles green synthesized using papaya extract in zebrafish.Biomedical reports · 2023Article
- Article
- Zinc Oxide Nanoparticles (ZnO-NPs) Induce Cytotoxicity in the Zebrafish Olfactory Organs via Activating Oxidative Stress and Apoptosis at the Ultrastructure and Genetic Levels.Animals : an open access journal from MDPI · 2023Article
- Protective potential of fresh orange juice against zinc oxide nanoparticles-induced trans-placental and trans-generational toxicity in mice.Food science & nutrition · 2023Article
- Acute Developmental Toxicity of Panax notoginseng in Zebrafish Larvae.Chinese journal of integrative medicine · 2023Article
- Application of transgenic zebrafish for investigating inflammatory responses to nanomaterials: Recommendations for new users.F1000Research · 2023Review
- Article
- Beneficial and toxicological aspects of zinc oxide nanoparticles in animals.Veterinary medicine and science · 2022Review
- ZnO nanostructured materials and their potential applications: progress, challenges and perspectives.Nanoscale advances · 2022Review
- A New Look at the Effects of Engineered ZnO and TiONanomaterials (Basel, Switzerland) · 2022Review
- Effects of Metal Oxide Nanoparticles in Zebrafish.Oxidative medicine and cellular longevity · 2022Review
- Article
- Molecular characterization of superoxide dismutase and catalase genes, and the induction of antioxidant genes under the zinc oxide nanoparticle-induced oxidative stress in air-breathing magur catfish (Clarias magur).Fish physiology and biochemistry · 2021Article
- The toxicity analysis of PVP, PVA and PEG surface functionalized ZnO nanoparticles on embryonic as well as adult Danio rerio.Environmental monitoring and assessment · 2021Article
- A Review on Conventional and Advanced Methods for Nanotoxicology Evaluation of Engineered Nanomaterials.Molecules (Basel, Switzerland) · 2021Review
- Changes of Gene Expression Patterns from Aquatic Organisms Exposed to Metal Nanoparticles.International journal of environmental research and public health · 2021Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Zinc oxide nanoparticles (ZnO NPs) are being utilized in an increasing number of fields and commercial applications. While their general toxicity and associated oxidative stress have been extensively studied, the toxicological pathways that they induce in developmental stages are still largely unknown. In this study, the developmental toxicity of ZnO NPs to embryonic/larval zebrafish was investigated. The transcriptional expression profiles induced by ZnO NPs were also investigated to ascertain novel genomic responses related to their specific toxicity pathway. Zebrafish embryos were exposed to 0.01, 0.1, 1, and 10 mg/L ZnO NPs for 96 h post-fertilization. The toxicity of ZnO NPs, based on their Zn concentration, was quite similar to that in embryonic/larval zebrafish exposed to corresponding ZnSO4 concentrations. Pericardial edema and yolk-sac edema were the principal malformations induced by ZnO NPs. Gene-expression profiling using microarrays demonstrated 689 genes that were differentially regulated (fold change >1.5) following exposure to ZnO NPs (498 upregulated, 191 downregulated). Several genes that were differentially regulated following ZnO NP exposure shared similar biological pathways with those observed with ZnSO4 exposure, but six genes (aicda, cyb5d1, edar, intl2, ogfrl2 and tnfsf13b) associated with inflammation and the immune system responded specifically to ZnO NPs (either in the opposite direction or were unchanged in ZnSO4 exposure). Real-time reverse-transcription quantitative polymerase chain reaction confirmed that the responses of these genes to ZnO NPs were significantly different from their response to ZnSO4 exposure. ZnO NPs may affect genes related to inflammation and the immune system, resulting in yolk-sac edema and pericardia edema in embryonic/larval developmental stages. These results will assist in elucidating the mechanisms of toxicity of ZnO NPs during development of zebrafish.
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