ArticleProceedings of the National Academy of Sciences of the United States of America2016
Regulatory evolution of Tbx5 and the origin of paired appendages.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 61 citations in OpenAlex.
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- Limb positioning and initiation: An evolutionary context of pattern and formation.Developmental dynamics : an official publication of the American Association of Anatomists · 2021Review
- The bowfin genome illuminates the developmental evolution of ray-finned fishes.Nature genetics · 2021Article
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- Exploring the Expression of Cardiac Regulators in a Vertebrate Extremophile: The Cichlid FishJournal of developmental biology · 2020Article
- Developmental constraints on fin diversity.Development, growth & differentiation · 2020Review
- Gene Regulatory and Expression Differences between Mouse and Pig Limb Buds Provide Insights into the Evolutionary Emergence of Artiodactyl Traits.Cell reports · 2020Article
- The Role of Retinoic Acid in Establishing the Early Limb Bud.Biomolecules · 2020Review
- Inference of the ancestral vertebrate phenotype through vestiges of the whole-genome duplications.Briefings in functional genomics · 2018Review
- Genomic Knockout of Two Presumed Forelimb Tbx5 Enhancers Reveals They Are Nonessential for Limb Development.Cell reports · 2018Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The diversification of paired appendages has been a major factor in the evolutionary radiation of vertebrates. Despite its importance, an understanding of the origin of paired appendages has remained elusive. To address this problem, we focused on T-box transcription factor 5 (Tbx5), a gene indispensable for pectoral appendage initiation and development. Comparison of gene expression in jawless and jawed vertebrates reveals that the Tbx5 expression in jawed vertebrates is derived in having an expression domain that extends caudal to the heart and gills. Chromatin profiling, phylogenetic footprinting, and functional assays enabled the identification of a Tbx5 fin enhancer associated with this apomorphic pattern of expression. Comparative functional analysis of reporter constructs reveals that this enhancer activity is evolutionarily conserved among jawed vertebrates and is able to rescue the finless phenotype of tbx5a mutant zebrafish. Taking paleontological evidence of early vertebrates into account, our results suggest that the gain of apomorphic patterns of Tbx5 expression and regulation likely contributed to the morphological transition from a finless to finned condition at the base of the vertebrate lineage.
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