ArticleParasites & vectors2016
Stimulation and quantification of Babesia divergens gametocytogenesis.
Article in Parasites & vectors, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 15 citations in OpenAlex.
- A Set of Rapid Diagnostic Tool for Babesia microti Infection.Journal of clinical laboratory analysis · 2025Article
- Theileria lestoquardi and malignant theileriosis in small ruminants: an updated review.Tropical animal health and production · 2025Review
- Developing Anti-International journal of molecular sciences · 2023Review
- Establishment of a stable transfection and gene targeting system inFrontiers in cellular and infection microbiology · 2023Article
- Extraordinary high level of propagation ofParasitology · 2022Article
- Direct injection of Amblyomma americanum ticks with Cytauxzoon felis.Ticks and tick-borne diseases · 2022Article
- A Culture-Adapted Strain ofFrontiers in cellular and infection microbiology · 2022Article
- Plasmepsin-like Aspartyl Proteases inPathogens (Basel, Switzerland) · 2021Article
- A framework for signaling throughout the life cycle of Babesia species.Molecular microbiology · 2021Review
- The Complexity of Piroplasms Life Cycles.Frontiers in cellular and infection microbiology · 2018Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBabesia divergens is the most common blood parasite in Europe causing babesiosis, a tick-borne malaria-like disease. Despite an increasing focus on B. divergens, especially regarding veterinary and human medicine, the sexual development of Babesia is poorly understood. Development of Babesia sexual stages in the host blood (gametocytes) plays a decisive role in parasite acquisition by the tick vector. However, the exact mechanism of gametocytogenesis is still unexplained.
methodsBabesia divergens gametocytes are characterized by expression of bdccp1, bdccp2 and bdccp3 genes. Using previously described sequences of bdccp1, bdccp2 and bdccp3, we have established a quantitative real-time PCR (qRT-PCR) assay for detection and assessment of the efficiency of B. divergens gametocytes production in bovine blood. We analysed fluctuations in expression of bdccp genes during cultivation in vitro, as well as in cultures treated with different drugs and stimuli.
resultsWe demonstrated that all B. divergens clonal lines tested, originally derived from naturally infected cows, exhibited sexual stages. Furthermore, sexual commitment was stimulated during continuous growth of the cultures, by addition of specific stress-inducing drugs or by alternating cultivation conditions. Expression of bdccp genes was greatly reduced or even lost after long-term cultivation, suggesting possible problems in the artificial infections of ticks in feeding assays in vitro.
conclusionsOur research provides insight into sexual development of B. divergens and may facilitate the development of transmission models in vitro, enabling a more detailed understanding of Babesia-tick interactions.
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