ArticleeLife2016
Conserved functional antagonism of CELF and MBNL proteins controls stem cell-specific alternative splicing in planarians.
Article in eLife, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
36 citing papers in PubMed, 73 citations in OpenAlex.
- An in vivo parallelized reporter assay to uncover tissue-specific splicing regulatory sequences in a multicellular animal.Nucleic acids research · 2026Article
- MBNL proteins in health, disease, and therapeutic applications.Nucleic acids research · 2026Review
- Multimodal single cell analyses reveal gene networks of planarian stem cell differentiation.Nature communications · 2025Article
- Alternative splicing in stem cells and development: research progress and emerging technologies.Cell regeneration (London, England) · 2025Review
- Functions of the Muscleblind-like protein family and their role in disease.Cell communication and signaling : CCS · 2025Review
- Stem cells (neoblasts) and positional information jointly dominate regeneration in planarians.Heliyon · 2025Review
- Fibrillarin homologs regulate translation in divergent cell lineages during planarian homeostasis and regeneration.The EMBO journal · 2024Article
- Robotic microinjection enables large-scale transgenic studies of Caenorhabditis elegans.Nature communications · 2024Article
- Novel insights into the potential applications of stem cells in pulmonary hypertension therapy.Respiratory research · 2024Review
- The known, unknown, and unknown unknowns of cell-cell communication in planarian regeneration.Zoological research · 2023Review
- RBPMS2 Is a Myocardial-Enriched Splicing Regulator Required for Cardiac Function.Circulation research · 2022Article
- Single-cell transcriptomics in planaria: new tools allow new insights into cellular and evolutionary features.Biochemical Society transactions · 2022Review
- Bookend: precise transcript reconstruction with end-guided assembly.Genome biology · 2022Article
- A developmentally programmed splicing failure contributes to DNA damage response attenuation during mammalian zygotic genome activation.Science advances · 2022Article
- The X-linked splicing regulator MBNL3 has been co-opted to restrict placental growth in eutherians.PLoS biology · 2022Article
- A pan-metazoan concept for adult stem cells: the wobbling Penrose landscape.Biological reviews of the Cambridge Philosophical Society · 2022Article
- Computational Analysis of Alternative Splicing Using VAST-TOOLS and the VastDB Framework.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Decoding Stem Cells: An Overview on Planarian Stem Cell Heterogeneity and Lineage Progression.Biomolecules · 2021Review
- ExOrthist: a tool to infer exon orthologies at any evolutionary distance.Genome biology · 2021Article
- Dynamics of alternative splicing during somatic cell reprogramming reveals functions for RNA-binding proteins CPSF3, hnRNP UL1, and TIA1.Genome biology · 2021Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 3 countries.
Funding
Abstract
In contrast to transcriptional regulation, the function of alternative splicing (AS) in stem cells is poorly understood. In mammals, MBNL proteins negatively regulate an exon program specific of embryonic stem cells; however, little is known about the in vivo significance of this regulation. We studied AS in a powerful in vivo model for stem cell biology, the planarian Schmidtea mediterranea. We discover a conserved AS program comprising hundreds of alternative exons, microexons and introns that is differentially regulated in planarian stem cells, and comprehensively identify its regulators. We show that functional antagonism between CELF and MBNL factors directly controls stem cell-specific AS in planarians, placing the origin of this regulatory mechanism at the base of Bilaterians. Knockdown of CELF or MBNL factors lead to abnormal regenerative capacities by affecting self-renewal and differentiation sets of genes, respectively. These results highlight the importance of AS interactions in stem cell regulation across metazoans.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.