ArticleMolecular biology and evolution2016
Survival and Evolution of a Large Multidrug Resistance Plasmid in New Clinical Bacterial Hosts.
Article in Molecular biology and evolution, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 147 papers.
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Who cites it
147 citing papers in PubMed, 245 citations in OpenAlex.
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- Mobile genetic elements drive a plasmid fusion and deletion lifecycle shaping evolution and antimicrobial resistance.Nature communications · 2026Article
- Systems engineering of engineered live biotherapeutics: A discovery-to-translation framework for streamlining microbiome therapeutic development.Journal of controlled release : official journal of the Controlled Release Society · 2026Review
- A fitness advantage from the pLVPK plasmid fuels the global spread of a carbapenem-resistant hypervirulent Klebsiella pneumoniae high-risk clone: ST11-KL64.International microbiology : the official journal of the Spanish Society for Microbiology · 2026Article
- Genomic characterization of KPC-2 and NDM coproducing carbapenem-resistantMicrobiology spectrum · 2026Article
- A dual-tier plasmid network model underpins the evolutionary success of pandemic Klebsiella pneumoniae ST11.Scientific reports · 2026Article
- Carbapenem-ResistantAntibiotics (Basel, Switzerland) · 2026Review
- Plasmid-Driven Resistome Diversity in 9700Antibiotics (Basel, Switzerland) · 2026Article
- Genomic perplexity and the evolution of context-dependent function.Molecular biology and evolution · 2026Article
- Evolution and rise of antimicrobial resistance in key public health bacteria within and across the One Health sectors.Future microbiology · 2026Review
- The plasmid-host fitness landscape: a new paradigm for predicting the fate of mobile resistance.Applied and environmental microbiology · 2026Review
- Eco-evolutionary responses to plasmid-dependent phage constrain the spread of multidrug-resistance plasmids.The ISME journal · 2026Article
- Transfer potential of F-like plasmids inISME communications · 2026Article
- Dissecting pOXA-48 fitness effects in clinical Enterobacterales using plasmid-wide CRISPRi screens.Nature communications · 2025Article
- Plasmid-driven strategies for clone success in Escherichia coli.Nature communications · 2025Article
- Evolution of a Plasmid Regulatory Circuit Ameliorates Plasmid Fitness Cost.Molecular biology and evolution · 2025Article
- Article
- Mobile genetic elements: the hidden puppet masters underlying infant gut microbiome assembly?Microbiome research reports · 2025Article
- A chromosomal mutation is superior to a plasmid-encoded mutation for plasmid fitness cost compensation.PLoS biology · 2024Article
- High antimicrobial resistance rates and multidrug resistance inVeterinary world · 2024Article
87 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Large conjugative plasmids are important drivers of bacterial evolution and contribute significantly to the dissemination of antibiotic resistance. Although plasmid borne multidrug resistance is recognized as one of the main challenges in modern medicine, the adaptive forces shaping the evolution of these plasmids within pathogenic hosts are poorly understood. Here we study plasmid-host adaptations following transfer of a 73 kb conjugative multidrug resistance plasmid to naïve clinical isolates of Klebsiella pneumoniae and Escherichia coli. We use experimental evolution, mathematical modelling and population sequencing to show that the long-term persistence and molecular integrity of the plasmid is highly influenced by multiple factors within a 25 kb plasmid region constituting a host-dependent burden. In the E. coli hosts investigated here, improved plasmid stability readily evolves via IS26 mediated deletions of costly regions from the plasmid backbone, effectively expanding the host-range of the plasmid. Although these adaptations were also beneficial to plasmid persistence in a naïve K. pneumoniae host, they were never observed in this species, indicating that differential evolvability can limit opportunities of plasmid adaptation. While insertion sequences are well known to supply plasmids with adaptive traits, our findings suggest that they also play an important role in plasmid evolution by maintaining the plasticity necessary to alleviate plasmid-host constrains. Further, the observed evolutionary strategy consistently followed by all evolved E. coli lineages exposes a trade-off between horizontal and vertical transmission that may ultimately limit the dissemination potential of clinical multidrug resistance plasmids in these hosts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.