ArticleRapid communications in mass spectrometry : RCM2016
Measurement of the accurate mass of a 50 MDa infectious virus.
Article in Rapid communications in mass spectrometry : RCM, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 53 citations in OpenAlex.
- Application of Gas-Phase Electrophoresis (nES GEMMA Instrumentation) in Molecular Weight Determination.Journal of mass spectrometry : JMS · 2025Review
- Diamond Molecular Balance: Ultra-Wide Range Nanomechanical Mass Spectrometry from MDa to TDa.Nano letters · 2025Article
- Standardized workflow for multiplexed charge detection mass spectrometry on orbitrap analyzers.Nature protocols · 2025Review
- Mass spectrometry-intensive top-down proteomics: an update on technology advancements and biomedical applications.Analytical methods : advancing methods and applications · 2024Review
- Square Membrane Resonators Supporting Degenerate Modes of Vibration for High-Throughput Mass Spectrometry of Single Bacterial Cells.ACS sensors · 2023Article
- High-throughput determination of dry mass of single bacterial cells by ultrathin membrane resonators.Communications biology · 2022Article
- Applications of Charge Detection Mass Spectrometry in Molecular Biology and Biotechnology.Chemical reviews · 2022Review
- Article
- Intravirion DNA Can Access the Space Occupied by the Bacteriophage P22 Ejection Proteins.Viruses · 2021Article
- Direct observation of ion emission from charged aqueous nanodrops: effects on gaseous macromolecular charging.Chemical science · 2021Article
- Mass Spectrometry-Based Structural Virology.Analytical chemistry · 2021Review
- Higher Resolution Charge Detection Mass Spectrometry.Analytical chemistry · 2020Article
- Individual Ion Mass Spectrometry Enhances the Sensitivity and Sequence Coverage of Top-Down Mass Spectrometry.Journal of proteome research · 2020Article
- Charge Detection Mass Spectrometry Measurements of Exosomes and other Extracellular Particles Enriched from Bovine Milk.Analytical chemistry · 2020Article
- Dramatic Improvement in Sensitivity with Pulsed Mode Charge Detection Mass Spectrometry.Analytical chemistry · 2019Article
- Virus-like particle size and molecular weight/mass determination applying gas-phase electrophoresis (native nES GEMMA).Analytical and bioanalytical chemistry · 2019Article
- Dissecting the Components of Sindbis Virus from Arthropod and Vertebrate Hosts: Implications for Infectivity Differences.ACS infectious diseases · 2019Article
- Real-Time Analysis and Signal Optimization for Charge Detection Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2019Article
- The FUNPET-a New Hybrid Ion Funnel-Ion Carpet Atmospheric Pressure Interface for the Simultaneous Transmission of a Broad Mass Range.Journal of the American Society for Mass Spectrometry · 2018Article
- Optimized Electrostatic Linear Ion Trap for Charge Detection Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2018Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
rationaleBacteriophage P22 is believed to contain a total of 521 copies of 9 different proteins and a 41,724 base pair genome. Despite its enormous size and complexity, phage P22 can be electrosprayed, and it remains intact in ultra-high vacuum where its molar mass distribution has been measured.
methodsPhage P22 virions were generated by complementation in Salmonella enterica and purified. They were transferred into 100 mM ammonium acetate and then electrosprayed. The masses of individual virions were determined using charge detection mass spectrometry.
resultsThe stoichiometry of the protein components of phage P22 is sufficiently well known that the theoretical molar mass can be determined to within a narrow range. The measured average molar mass of phage P22, 52,180 ± 59 kDa, is consistent with the theoretical molar mass and supports the proposed stoichiometry of the components. The intrinsic width of the phage P22 mass distribution can be accounted for by the distribution of DNA packaged by the headful mechanism.
conclusionsAt over 50 MDa, phage P22 is the largest object with a well-defined molar mass to be analyzed by mass spectrometry. The narrow measured mass distribution indicates that the virions survive the transition into the gas phase intact. Copyright © 2016 John Wiley & Sons, Ltd.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.