ArticlePloS one2016
In Vivo Cannulation Methods for Cardiomyocytes Isolation from Heart Disease Models.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 14 citations in OpenAlex.
- Guidelines for experimental models of myocardial ischemia and infarction.American journal of physiology. Heart and circulatory physiology · 2018Guideline
- Modified langendorff-free method for isolating cardiomyocytes and non-cardiomyocytes from adult mice.BMC biotechnology · 2026Article
- A modification of retrograde Langendorff-perfusion to increase the yield of CaFrontiers in pharmacology · 2026Article
- Distinct morphometric features of cardiomyocytes isolated from mouse hypertrophy models: An ImageJ analysis combined with machine learning algorithms.Physiological reports · 2025Article
- A Simple and Effective Method to Consistently Isolate Mouse Cardiomyocytes.Journal of visualized experiments : JoVE · 2022Article
- Protocol to record and quantify the intracellular pH in contracting cardiomyocytes.STAR protocols · 2022Article
- Article
- A Model for Studying the Biomechanical Effects of Varying Ratios of Collagen Types I and III on Cardiomyocytes.Cardiovascular engineering and technology · 2021Article
- Amorphous SiO2 nanoparticles promote cardiac dysfunction via the opening of the mitochondrial permeability transition pore in rat heart and human cardiomyocytes.Particle and fibre toxicology · 2020Article
- A Microwell Cell Capture Device Reveals Variable Response to Dobutamine in Isolated Cardiomyocytes.Biophysical journal · 2019Article
- Illuminating cell signaling with genetically encoded FRET biosensors in adult mouse cardiomyocytes.The Journal of general physiology · 2018Article
- Modified Langendorff technique for mouse heart cannulation: Improved heart quality and decreased risk of ischemia.MethodsX · 2017Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
Isolation of high quality cardiomyocytes is critically important for achieving successful experiments in many cellular and molecular cardiology studies. Methods for isolating cardiomyocytes from the murine heart generally are time-sensitive and experience-dependent, and often fail to produce high quality cells. Major technical difficulties can be related to the surgical procedures needed to explant the heart and to cannulate the vessel to mount onto the Langendorff system before in vitro reperfusion can begin. During this period, transient hypoxia and ischemia may damage the heart, resulting in low yield and poor quality of cells, especially for heart disease models that have fragile cells. We have developed novel in vivo cannulation methods to minimize hypoxia and ischemia, and fine-tuned the entire protocol to produce high quality ventricular myocytes. The high cell quality has been confirmed using important structural and functional criteria such as morphology, t-tubule structure, action potential morphology, Ca2+ signaling, responsiveness to beta-adrenergic agonist, and ability to have robust contraction under mechanically loaded condition. Together these assessments show the preservation of the cardiac excitation-contraction machinery in cells isolated using this technique. The in vivo cannulation method enables consistent isolation of high-quality cardiomyocytes, even from heart disease models that were notoriously difficult for cell isolation using traditional methods.
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Registered trials
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