ArticlePloS one2016
Transgenic Suppression of AGAMOUS Genes in Apple Reduces Fertility and Increases Floral Attractiveness.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 37 citations in OpenAlex.
- Flowering pathway genes: key targets for accelerated breeding in woody plants.Plant cell reports · 2025Review
- Mini review: Apple improvement, traditional approaches, biotechnology options, and regulatory considerations.Frontiers in bioengineering and biotechnology · 2025Review
- Apple CRISPR-Cas9-A Recipe for Successful Targeting ofPlants (Basel, Switzerland) · 2023Article
- Variation in floral form of CRISPR knock-outs of the poplar homologs ofHorticulture research · 2023Article
- Article
- The Roles of Floral Organ Genes in Regulating Rosaceae Fruit Development.Frontiers in plant science · 2021Review
- RNAi of AGAMOUS genes in sweetgum alters reproductive organ identity and decreases fruit persistence.Plant direct · 2020Article
- The Artificial PromoterInternational journal of molecular sciences · 2019Article
- RNA interference suppression of AGAMOUS and SEEDSTICK alters floral organ identity and impairs floral organ determinacy, ovule differentiation, and seed-hair development in Populus.The New phytologist · 2019Article
- Expression and functional analysis of applePlant biotechnology (Tokyo, Japan) · 2018Article
- Combination ofHorticulture research · 2017Article
- Genome-wide identification of thePeerJ · 2017Article
- New Biotechnological Tools for the Genetic Improvement of Major Woody Fruit Species.Frontiers in plant science · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the ability of RNA interference (RNAi) directed against two co-orthologs of AGAMOUS (AG) from Malus domestica (domestic apple, MdAG) to reduce the risks of invasiveness and provide genetic containment of transgenes, while also promoting the attractiveness of flowers for ornamental usage. Suppression of two MdAG-like genes, MdMADS15 and MdMADS22, led to the production of trees with highly showy, polypetalous flowers. These "double-flowers" had strongly reduced expression of both MdAG-like genes. Members of the two other clades within in the MdAG subfamily showed mild to moderate differences in gene expression, or were unchanged, with the level of suppression approximately proportional to the level of sequence identity between the gene analyzed and the RNAi fragment. The double-flowers also exhibited reduced male and female fertility, had few viable pollen grains, a decreased number of stigmas, and produced few viable seeds after cross-pollination. Despite these floral alterations, RNAi-AG trees with double-flowers set full-sized fruit. Suppression or mutation of apple AG-like genes appears to be a promising method for combining genetic containment with improved floral attractiveness.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.