ArticleJournal of visualized experiments : JoVE2016
Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS.
Article in Journal of visualized experiments : JoVE, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Comprehensive Phenotypic Characterization of ClinicalInternational journal of molecular sciences · 2026Article
- Comparison of three routine methods for the identification ofFrontiers in microbiology · 2026Article
- Review
- Review
- Review
- The Oral Microbiome and Systemic Health: Bridging the Gap Between Dentistry and Medicine.Cureus · 2025Review
- Variations in bacterial profiles associated with semen collection timing and bull breed, analyzed using 16S rRNA sequencing and MALDI-TOF MS.Frontiers in veterinary science · 2025Article
- Current knowledge on Inquilinus limosus, a scarcely researched human pathogen.BMC microbiology · 2024Review
- Expression profiles of sporulation genes in multidrug-resistantExperimental biology and medicine (Maywood, N.J.) · 2023Article
- Targeted Sequencing Approach and Its Clinical Applications for the Molecular Diagnosis of Human Diseases.Cells · 2023Review
- Comparative Study of Different Diagnostic Routine Methods for the Identification ofMicroorganisms · 2022Article
- Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry.Journal of visualized experiments : JoVE · 2022Article
- Bacteriospermia - A formidable player in male subfertility.Open life sciences · 2022Review
- Atypical pathogens associated with cardiac implantable electronic device infections.Pacing and clinical electrophysiology : PACE · 2021Article
- Identification and Antibiotic Profiling ofFrontiers in microbiology · 2021Article
- The heat shock protein 70 gene as a new alternative molecular marker for the taxonomic identification of Streptomyces strains.AMB Express · 2018Article
- Whole-genome sequencing of a large collection of Myroides odoratimimus and Myroides odoratus isolates and antimicrobial susceptibility studies.Emerging microbes & infections · 2018Article
- A case ofJMM case reports · 2017Article
- Wohlfahrtiimonas chitiniclastica: current insights into an emerging human pathogen.Epidemiology and infection · 2017Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There are a number of rare and, therefore, insufficiently described bacterial pathogens which are reported to cause severe infections especially in immunocompromised patients. In most cases only few data, mostly published as case reports, are available which investigate the role of such pathogens as an infectious agent. Therefore, in order to clarify the pathogenic character of such microorganisms, it is necessary to conduct epidemiologic studies which include large numbers of these bacteria. The methods used in such a surveillance study have to meet the following criteria: the identification of the strains has to be accurate according to the valid nomenclature, they should be easy to handle (robustness), economical in routine diagnostics and they have to generate comparable results among different laboratories. Generally, there are three strategies for identifying bacterial strains in a routine setting: 1) phenotypic identification characterizing the biochemical and metabolic properties of the bacteria, 2) molecular techniques such as 16S rRNA gene sequencing and 3) mass spectrometry as a novel proteome based approach. Since mass spectrometry and molecular approaches are the most promising tools for identifying a large variety of bacterial species, these two methods are described. Advances, limitations and potential problems when using these techniques are discussed.
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