ArticlePloS one2016
A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate Expression Constructs with Diverse Research Applications.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Article
- Genetic interrogation reveals mechanisms regulating nexus glia development and shaping heart physiology.Developmental cell · 2026Article
- Targeted recruitment of USP15 enhances CTLA4 surface levels and restricts its degradation.Life science alliance · 2026Article
- MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible cloning toolbox for use in vertebrate model systems.Development (Cambridge, England) · 2025Article
- Imaging cellular activity simultaneously across all organs of a vertebrate reveals body-wide circuits.bioRxiv : the preprint server for biology · 2025Article
- Defining the molecular identity and morphology of glia limitans superficialis astrocytes in vertebrates.Cell reports · 2025Article
- Neuropeptidergic regulation of neuromuscular signaling in larval zebrafish alters swimming behavior and synaptic transmission.iScience · 2024Article
- Lineage-tracing hematopoietic stem cell origins in vivo to efficiently make human HLF+ HOXA+ hematopoietic progenitors from pluripotent stem cells.Developmental cell · 2024Article
- Refining the genomic determinants underlying escape from X-chromosome inactivation.NAR genomics and bioinformatics · 2023Article
- The CRISPR-Cas12a Platform for Accurate Genome Editing, Gene Disruption, and Efficient Transgene Integration in Human Immune Cells.ACS synthetic biology · 2023Article
- The dependence of EGFR oligomerization on environment and structure: A camera-based N&B study.Biophysical journal · 2022Article
- Imaging Mitochondrial Dynamics in theCold Spring Harbor protocols · 2021Article
- Cocaine Triggers Astrocyte-Mediated Synaptogenesis.Biological psychiatry · 2021Article
- Suppression of breast cancer cells resistant to a pure anti-estrogen with CAR-transduced natural killer cells.American journal of cancer research · 2021Article
- Quantifying protein dynamics and stability in a living organism.Nature communications · 2019Article
- Neural stem cell quiescence and stemness are molecularly distinct outputs of the Notch3 signalling cascade in the vertebrate adult brain.Development (Cambridge, England) · 2018Article
- Redundant Postsynaptic Functions of SynCAMs 1-3 during Synapse Formation.Frontiers in molecular neuroscience · 2017Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors.
Funding
Abstract
Recombination-based cloning is a quick and efficient way to generate expression vectors. Recent advancements have provided powerful recombinant DNA methods for molecular manipulations. Here, we describe a novel collection of three-fragment MultiSite Gateway cloning system-compatible vectors providing expanded molecular tools for vertebrate research. The components of this toolkit encompass a broad range of uses such as fluorescent imaging, dual gene expression, RNA interference, tandem affinity purification, chemically-inducible dimerization and lentiviral production. We demonstrate examples highlighting the utility of this toolkit for producing multi-component vertebrate expression vectors with diverse primary research applications. The vectors presented here are compatible with other Gateway toolkits and collections, facilitating the rapid generation of a broad range of innovative DNA constructs for biological research.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.