ArticleJournal of visualized experiments : JoVE2016
Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions.
Article in Journal of visualized experiments : JoVE, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 91 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
91 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Meta-analysis suggests the microbiome responds to Evolve and Resequence experiments in Drosophila melanogaster.BMC microbiology · 2021Pooled it
- Microbiome-mediated resilience and cross-generational consequences in male Drosophila exposed to combined environmental stressors.Proceedings. Biological sciences · 2026Article
- A Landscape ofBiology · 2026Review
- Environmental microbial extracts for longitudinal studies of gut microbiome assembly and maintenance.bioRxiv : the preprint server for biology · 2026Article
- From diet to function: using synthetic microbial communities to map gut microbial interactions.NPJ biofilms and microbiomes · 2026Review
- Microbe-dependent inter-organ communication regulates germline stem cell proliferation inbioRxiv : the preprint server for biology · 2026Article
- Review
- Caenorhabditis elegans as an experimental model for resilience-boosting microbiota interventions in nonmodel ectotherms.FEMS microbiology reviews · 2026Review
- Altered mitochondrial functionality in metabolic disorders: insights fromFrontiers in endocrinology · 2026Review
- Systematic analysis of nutrient-microbiome interactions and their effects on host phenotypes inmBio · 2025Article
- Gut Microbial Genetic Variation Prolongs Host Healthy Longevity and Remodels Metabolome and Proteome in Drosophila Melanogaster.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Whole-body homogenates restore disrupted microbiota composition in a model insect better than feces or no restoration treatment.bioRxiv : the preprint server for biology · 2025Article
- Is it time to assignApplied and environmental microbiology · 2025Article
- The microbiota elicits compensatory adaptation in a seasonally-adapting animal host.bioRxiv : the preprint server for biology · 2025Article
- Symbiotic bacteria participate in pectinolytic metabolism to enhance larval growth in Zeugodacus cucurbitae.Pest management science · 2025Article
- A rationally designed microbial consortium modulates neurodegeneration in a Drosophila melanogaster model of Parkinson's disease.NPJ biofilms and microbiomes · 2025Article
- Structure, function, and quantitative biology of the Drosophila gut microbiome.Current opinion in microbiology · 2025Review
- Microbiome evolution plays a secondary role in host rapid adaptation.bioRxiv : the preprint server for biology · 2025Article
- Translocation of gut bacteria promotes tumor-associated mortality by inducing immune-activated renal damage.The EMBO journal · 2025Article
- Gut microbiome and host TOR pathway interact to regulate predator-induced aversive memory inProceedings of the National Academy of Sciences of the United States of America · 2025Article
31 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
Abstract
The influence of microbes on myriad animal traits and behaviors has been increasingly recognized in recent years. The fruit fly Drosophila melanogaster is a model for understanding microbial interactions with animal hosts, facilitated by approaches to rear large sample sizes of Drosophila under microorganism-free (axenic) conditions, or with defined microbial communities (gnotobiotic). This work outlines a method for collection of Drosophila embryos, hypochlorite dechorionation and sterilization, and transfer to sterile diet. Sterilized embryos are transferred to sterile diet in 50 ml centrifuge tubes, and developing larvae and adults remain free of any exogenous microbes until the vials are opened. Alternatively, flies with a defined microbiota can be reared by inoculating sterile diet and embryos with microbial species of interest. We describe the introduction of 4 bacterial species to establish a representative gnotobiotic microbiota in Drosophila. Finally, we describe approaches for confirming bacterial community composition, including testing if axenic Drosophila remain bacteria-free into adulthood.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.