ArticleRegeneration (Oxford, England)2015
Positional information in axolotl and mouse limb extracellular matrix is mediated via heparan sulfate and fibroblast growth factor during limb regeneration in the axolotl (Ambystoma mexicanum).
Article in Regeneration (Oxford, England), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed, 66 citations in OpenAlex.
- Dynamic control of cell state transitions during tissue morphogenesis.The EMBO journal · 2026Review
- tBid-Mediated Genetic Ablation of Connective Tissue Cells Reveals Their Key Regulatory Function During Limb Regeneration in Axolotls.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Posterior shift of Shh-Fgf signaling in axolotl limb regeneration drives sequential digit formation.iScience · 2026Article
- The Accessory Limb Model Regenerative Assay and Its Derivatives.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Integration failure of regenerated limb tissue is associated with incongruencies in positional information in the Mexican axolotl.Frontiers in cell and developmental biology · 2023Article
- Toeing the line between regeneration and fibrosis.Frontiers in cell and developmental biology · 2023Review
- Overexpression of NDST1 Attenuates Fibrotic Response in Murine Adipose-Derived Stem Cells.Stem cells and development · 2022Article
- Manipulating the microbiome alters regenerative outcomes in Xenopus laevis tadpoles via lipopolysaccharide signalling.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society · 2022Article
- Article
- Positional Memory in Vertebrate Regeneration: A Century's Insights from the Salamander Limb.Cold Spring Harbor perspectives in biology · 2022Review
- Muscles are barely required for the patterning and cell dynamics in axolotl limb regeneration.Frontiers in genetics · 2022Article
- Neural regulation of body polarities in nereid worm regeneration.FASEB bioAdvances · 2022Article
- The MAPK Signaling Pathway Presents Novel Molecular Targets for Therapeutic Intervention after Traumatic Spinal Cord Injury: A Comparative Cross-Species Transcriptional Analysis.International journal of molecular sciences · 2021Article
- Retinoic Acid Receptors and the Control of Positional Information in the Regenerating Axolotl Limb.Cells · 2021Article
- Characterizing the regenerative capacity and growth patterns of the Texas blind salamander (Eurycea rathbuni).Developmental dynamics : an official publication of the American Association of Anatomists · 2021Article
- Big-Data Glycomics: Tools to Connect Glycan Biosynthesis to Extracellular Communication.Trends in biochemical sciences · 2021Review
- Decellularization Enables Characterization and Functional Analysis of Extracellular Matrix in Planarian Regeneration.Molecular & cellular proteomics : MCP · 2021Article
- ECM-mediated positional cues are able to induce pattern, but not new positional information, during axolotl limb regeneration.PloS one · 2021Article
- Identification of Heparan-Sulfate Rich Cells in the Loose Connective Tissues of the Axolotl (Ambystoma mexicanum) with the Potential to Mediate Growth Factor Signaling during Regeneration.Regenerative engineering and translational medicine · 2020Article
- Operation spinal cord regeneration: Patterning information residing in extracellular matrix glycosaminoglycans.Brain and behavior · 2020Review
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Urodele amphibians are unique among adult vertebrates in their ability to regenerate complex body structures after traumatic injury. In salamander regeneration, the cells maintain a memory of their original position and use this positional information to recreate the missing pattern. We used an in vivo gain-of-function assay to determine whether components of the extracellular matrix (ECM) have positional information required to induce formation of new limb pattern during regeneration. We discovered that salamander limb ECM has a position-specific ability to either inhibit regeneration or induce de novo limb structure, and that this difference is dependent on heparan sulfates that are associated with differential expression of heparan sulfate sulfotransferases. We also discovered that an artificial ECM containing only heparan sulfate was sufficient to induce de novo limb pattern in salamander limb regeneration. Finally, ECM from mouse limbs is capable of inducing limb pattern in axolotl blastemas in a position-specific, developmental-stage-specific, and heparan sulfate-dependent manner. This study demonstrates a mechanism for positional information in regeneration and establishes a crucial functional link between salamander regeneration and mammals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.