ArticleRegeneration (Oxford, England)2015
TALEN-mediated gene editing of the thrombospondin-1 locus in axolotl.
Article in Regeneration (Oxford, England), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 13 citations in OpenAlex.
- The use of transgenics in the laboratory axolotl.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Review
- Towards comparative analyses of salamander limb regeneration.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2021Review
- Integrative Analysis of Axolotl Gene Expression Data from Regenerative and Wound Healing Limb Tissues.Scientific reports · 2019Article
- Application and optimization of CRISPR-Cas9-mediated genome engineering in axolotl (Ambystoma mexicanum).Nature protocols · 2018Article
- Advances in Decoding Axolotl Limb Regeneration.Trends in genetics : TIG · 2017Review
- DiI Perfusion as a Method for Vascular Visualization in Ambystoma mexicanum.Journal of visualized experiments : JoVE · 2017Article
- Identification of Mutant Genes and Introgressed Tiger Salamander DNA in the Laboratory Axolotl, Ambystoma mexicanum.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Loss-of-function genetics provides strong evidence for a gene's function in a wild-type context. In many model systems, this approach has been invaluable for discovering the function of genes in diverse biological processes. Axolotls are urodele amphibians (salamanders) with astonishing regenerative abilities, capable of regenerating entire limbs, portions of the tail (including spinal cord), heart, and brain into adulthood. With their relatively short generation time among salamanders, they offer an outstanding opportunity to interrogate natural mechanisms for appendage and organ regeneration provided that the tools are developed to address these long-standing questions. Here we demonstrate targeted modification of the thrombospondin-1 (tsp-1) locus using transcription-activator-like effector nucleases (TALENs) and identify a role of tsp-1 in recruitment of myeloid cells during limb regeneration. We find that while tsp-1-edited mosaic animals still regenerate limbs, they exhibit a reduced subepidermal collagen layer in limbs and an increased number of myeloid cells within blastemas. This work presents a protocol for generating and genotyping mosaic axolotls with TALEN-mediated gene edits.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.