ArticleBiotechnology for biofuels2016
Exploring xylose metabolism in Spathaspora species: XYL1.2 from Spathaspora passalidarum as the key for efficient anaerobic xylose fermentation in metabolic engineered Saccharomyces cerevisiae.
Article in Biotechnology for biofuels, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 129 citations in OpenAlex.
- Biotechnological applications of D-xylose-fermenting autochthonous yeasts in lignocellulosic biorefineries.Biotechnology for biofuels and bioproducts · 2026Review
- Glucose receptor deletion and engineering: impact on xylose sensing and utilization in Saccharomyces cerevisiae.FEMS yeast research · 2025Article
- Machine learning enables identification of an alternative yeast galactose utilization pathway.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Evaluation of Pyrophosphate-Driven Proton Pumps inMicroorganisms · 2024Article
- Oxygenation influences xylose fermentation and gene expression in the yeast genera Spathaspora and Scheffersomyces.Biotechnology for biofuels and bioproducts · 2024Article
- Exploring xylose metabolism in non-conventional yeasts: kinetic characterization and product accumulation under different aeration conditions.Journal of industrial microbiology & biotechnology · 2024Article
- FromJournal of fungi (Basel, Switzerland) · 2023Review
- Codon Optimization Improves the Prediction of Xylose Metabolism from Gene Content in Budding Yeasts.Molecular biology and evolution · 2023Article
- Review
- Using phosphoglucose isomerase-deficient (pgi1Δ) Saccharomyces cerevisiae to map the impact of sugar phosphate levels on D-glucose and D-xylose sensing.Microbial cell factories · 2022Article
- Strategies for Efficient Expression of Heterologous Monosaccharide Transporters inJournal of fungi (Basel, Switzerland) · 2022Article
- Analysis of glucose and xylose metabolism in new indigenous Meyerozyma caribbica strains isolated from corn residues.World journal of microbiology & biotechnology · 2022Article
- Current Ethanol Production Requirements for the YeastInternational journal of microbiology · 2022Review
- D-Xylose Sensing inInternational journal of molecular sciences · 2021Review
- Performance of xylose-fermenting yeasts in oat and soybean hulls hydrolysate and improvement of ethanol production using immobilized cell systems.Biotechnology letters · 2021Article
- Bioethanolic yeasts from dung beetles: tapping the potential of extremophilic yeasts for improvement of lignocellulolytic feedstock fermentation.Biotechnology for biofuels · 2021Review
- EngineeredBioengineered · 2020Review
- Five new additions to the genusMycoKeys · 2020Article
- Genomic and transcriptomic analysis ofBiotechnology for biofuels · 2020Article
- Xylitol Production: Identification and Comparison of New Producing Yeasts.Microorganisms · 2019Article
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8 authors at 4 institutions in 4 countries.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundThe production of ethanol and other fuels and chemicals from lignocellulosic materials is dependent of efficient xylose conversion. Xylose fermentation capacity in yeasts is usually linked to xylose reductase (XR) accepting NADH as cofactor. The XR from Scheffersomyces stipitis, which is able to use NADH as cofactor but still prefers NADPH, has been used to generate recombinant xylose-fermenting Saccharomyces cerevisiae. Novel xylose-fermenting yeasts species, as those from the Spathaspora clade, have been described and are potential sources of novel genes to improve xylose fermentation in S. cerevisiae.
resultsXylose fermentation by six strains from different Spathaspora species isolated in Brazil, plus the Sp. passalidarum type strain (CBS 10155(T)), was characterized under two oxygen-limited conditions. The best xylose-fermenting strains belong to the Sp. passalidarum species, and their highest ethanol titers, yields, and productivities were correlated to higher XR activity with NADH than with NADPH. Among the different Spathaspora species, Sp. passalidarum appears to be the sole harboring two XYL1 genes: XYL1.1, similar to the XYL1 found in other Spathaspora and yeast species and XYL1.2, with relatively higher expression level. XYL1.1p and XYL1.2p from Sp. passalidarum were expressed in S. cerevisiae TMB 3044 and XYL1.1p was confirmed to be strictly NADPH-dependent, while XYL1.2p to use both NADPH and NADH, with higher activity with the later. Recombinant S. cerevisiae strains expressing XYL1.1p did not show anaerobic growth in xylose medium. Under anaerobic xylose fermentation, S. cerevisiae TMB 3504, which expresses XYL1.2p from Sp. passalidarum, revealed significant higher ethanol yield and productivity than S. cerevisiae TMB 3422, which harbors XYL1p N272D from Sc. stipitis in the same isogenic background (0.40 vs 0.34 g gCDW (-1) and 0.33 vs 0.18 g gCDW (-1) h(-1), respectively).
conclusionThis work explored a new clade of xylose-fermenting yeasts (Spathaspora species) towards the engineering of S. cerevisiae for improved xylose fermentation. The new S. cerevisiae TMB 3504 displays higher XR activity with NADH than with NADPH, with consequent improved ethanol yield and productivity and low xylitol production. This meaningful advance in anaerobic xylose fermentation by recombinant S. cerevisiae (using the XR/XDH pathway) paves the way for the development of novel industrial pentose-fermenting strains.
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