ArticleBiotechnology for biofuels2016
Evaluation of the two-step treatment with ionic liquids and alkali for enhancing enzymatic hydrolysis of Eucalyptus: chemical and anatomical changes.
Article in Biotechnology for biofuels, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 98 citations in OpenAlex.
- Enzymatic Production of Prebiotic Xylooligosaccharides Using aJournal of agricultural and food chemistry · 2026Article
- Navigating the nano-world future: Harnessing cellulose nanocrystals from green sources for sustainable innovation.Heliyon · 2025Review
- Efficacy and Functional Mechanisms of a Two-Stage Pretreatment Approach Based on Alkali and Ionic Liquid for Bioconversion of Waste Medium-Density Fiberboard.Molecules (Basel, Switzerland) · 2024Article
- Challenges in Using Ionic Liquids for Cellulosic Ethanol Production.Molecules (Basel, Switzerland) · 2023Review
- Surfactant-assisted alkaline pretreatment and enzymatic hydrolysis ofFrontiers in bioengineering and biotechnology · 2022Article
- Visualising lignin quantitatively in plant cell walls by micro-Raman spectroscopy.RSC advances · 2021Article
- Bioethanol production using high densityHeliyon · 2021Article
- A Two-Step Ferric Chloride and Dilute Alkaline Pretreatment for Enhancing Enzymatic Hydrolysis and Fermentable Sugar Recovery from Miscanthus sinensis.Molecules (Basel, Switzerland) · 2020Article
- Enhanced Enzymatic Hydrolysis ofMolecules (Basel, Switzerland) · 2019Article
- Enhanced co-generation of cellulosic ethanol and methane with the starch/sugar-rich waste mixtures and Tween 80 in fed-batch mode.Biotechnology for biofuels · 2019Article
- Treatment of Coffee Husk with Ammonium-Based Ionic Liquids: Lignin Extraction, Degradation, and Characterization.ACS omega · 2018Article
- Structural changes in lignocellulosic biomass during activation with ionic liquids comprising 3-methylimidazolium cations and carboxylate anions.Biotechnology for biofuels · 2018Article
- Characterization of the Micromorphology and Topochemistry of Poplar Wood during Mild Ionic Liquid Pretreatment for Improving Enzymatic Saccharification.Molecules (Basel, Switzerland) · 2017Article
- Biorefinery cascade processing for creating added value on tomato industrial by-products from Tunisia.Biotechnology for biofuels · 2016Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe biomass recalcitrance resulting from its chemical compositions and physical structures impedes the conversion of biomass into fermentable sugars. Pretreatment is a necessary procedure to increase the cellulase accessibility for bioconversion of lignocelluloses into bioethanol. Alternatively, ionic liquids, a series of promising solvents, provide unique opportunities for pretreating a wide range of lignocellulosic materials. In this study, a two-step treatment including ionic liquids pretreatment and successive alkali fractionations was performed on Eucalyptus to achieve a high enzymatic digestibility. The compositional and structural changes of Eucalyptus cell walls and their possible effect on saccharification ratio were comprehensively investigated.
resultsAfter the ionic liquids pretreatment, the cell walls became loose and even swelled, accompanying with the decrease of cellulose crystallinity. As compared to the simplex ionic liquids pretreatment, the integrated process resulted in the significant removal of hemicelluloses and lignin, enhancing the disruption of the cell walls and increasing the exposure of cellulose, which led to a higher conversion of cellulose to glucose. The glucose yield of Eucalyptus underwent the combination of [Bmim]OAc and alkali treatments reached the maximum (90.53 %), which was 6.6 times higher than that of the untreated Eucalyptus. The combination of chemical compositions and physical structure of Eucalyptus affected the efficiency of cellulose enzymatic hydrolysis. Especially, the changes of cellulose crystallinity played a major role in enhancing the enzymatic digestibility of Eucalyptus in this study.
conclusionsThe two-step treatment with ionic liquids pretreatment and successive alkali fractionation can be considered as a promising method to improve the conversion of cellulose to glucose. The detailed information obtained about chemical and anatomical changes was helpful to understand the underlying mechanism of the integrated treatment process acting on Eucalyptus for enhancing enzymatic digestibility.
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