Evidence map›Paper›PMID 27499617›Full record

ArticleBioinformatics and biology insights2016

OSG-GEM: Gene Expression Matrix Construction Using the Open Science Grid.

William L Poehlman, Mats Rynge, Chris Branton, D Balamurugan, Frank A Feltus

Abstract read
In one paragraph

Article in Bioinformatics and biology insights, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Moving Just Enough Deep Sequencing Data to Get the Job Done.Bioinformatics and biology insights · 2019
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

William L PoehlmanDepartment of Genetics and Biochemistry, Clemson University, SC, USA.
Mats RyngeInformation Sciences Institute, University of Southern California, Marina Del Rey, CA, USA.
Chris BrantonCenter for Computation and Technology, Louisiana State University, Baton Rouge, LA, USA.
D BalamuruganComputation Institute, University of Chicago, Chicago, IL, USA.
Frank A FeltusDepartment of Genetics and Biochemistry, Clemson University, SC, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

High-throughput DNA sequencing technology has revolutionized the study of gene expression while introducing significant computational challenges for biologists. These computational challenges include access to sufficient computer hardware and functional data processing workflows. Both these challenges are addressed with our scalable, open-source Pegasus workflow for processing high-throughput DNA sequence datasets into a gene expression matrix (GEM) using computational resources available to U.S.-based researchers on the Open Science Grid (OSG). We describe the usage of the workflow (OSG-GEM), discuss workflow design, inspect performance data, and assess accuracy in mapping paired-end sequencing reads to a reference genome. A target OSG-GEM user is proficient with the Linux command line and possesses basic bioinformatics experience. The user may run this workflow directly on the OSG or adapt it to novel computing environments.

Indexed as

distributed computingDNA sequence analysisOpen Science GridPegasusRNAseq

Identifiers

PMID27499617
PMCPMC4972127

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.