ArticleCell chemical biology2016
Global Cysteine-Reactivity Profiling during Impaired Insulin/IGF-1 Signaling in C. elegans Identifies Uncharacterized Mediators of Longevity.
Article in Cell chemical biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
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- Peroxiredoxin 2 is required for the redox mediated adaptation to exercise.Redox biology · 2023Article
- Emerging chemistry and biology in protein glutathionylation.Current opinion in chemical biology · 2022Review
- Recent Progress in Regulation of Aging by Insulin/IGF-1 Signaling inMolecules and cells · 2022Review
- CRISPR-based oligo recombineering prioritizes apicomplexan cysteines for drug discovery.Nature microbiology · 2022Article
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- Chemoproteomic interrogation of selenocysteine by low-pH isoTOP-ABPP.Methods in enzymology · 2022Article
- Global profiling of distinct cysteine redox forms reveals wide-ranging redox regulation in C. elegans.Nature communications · 2021Article
- A quantitative thiol reactivity profiling platform to analyze redox and electrophile reactive cysteine proteomes.Nature protocols · 2020Article
- Article
- Who Is Metabolizing What? Discovering Novel Biomolecules in the Microbiome and the Organisms Who Make Them.Frontiers in cellular and infection microbiology · 2020Article
- Chemical Biology Approaches to Interrogate the Selenoproteome.Accounts of chemical research · 2019Review
- Article
- The glutathione system and the related thiol network in Caenorhabditis elegans.Redox biology · 2019Review
- Pharmacological convergence reveals a lipid pathway that regulates C. elegans lifespan.Nature chemical biology · 2019Article
- Cytoplasmic and Mitochondrial NADPH-Coupled Redox Systems in the Regulation of Aging.Nutrients · 2019Review
- A Quantitative Chemoproteomic Platform to Monitor Selenocysteine Reactivity within a Complex Proteome.Cell chemical biology · 2018Article
- Dynamic redox balance directs the oocyte-to-embryo transition via developmentally controlled reactive cysteine changes.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
In the nematode Caenorhabditis elegans, inactivating mutations in the insulin/IGF-1 receptor, DAF-2, result in a 2-fold increase in lifespan mediated by DAF-16, a FOXO-family transcription factor. Downstream protein activities that directly regulate longevity during impaired insulin/IGF-1 signaling (IIS) are poorly characterized. Here, we use global cysteine-reactivity profiling to identify protein activity changes during impaired IIS. Upon confirming that cysteine reactivity is a good predictor of functionality in C. elegans, we profiled cysteine-reactivity changes between daf-2 and daf-16;daf-2 mutants, and identified 40 proteins that display a >2-fold change. Subsequent RNAi-mediated knockdown studies revealed that lbp-3 and K02D7.1 knockdown caused significant increases in lifespan and dauer formation. The proteins encoded by these two genes, LBP-3 and K02D7.1, are implicated in intracellular fatty acid transport and purine metabolism, respectively. These studies demonstrate that cysteine-reactivity profiling can be complementary to abundance-based transcriptomic and proteomic studies, serving to identify uncharacterized mediators of C. elegans longevity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.