ArticleScientific reports2016
Simplified three-dimensional tissue clearing and incorporation of colorimetric phenotyping.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed.
- Refractive Index-Corrected Light-Sheet Microscopy for Macro-View Cardiovascular Imaging.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Evaluation of the effects of clearing agents, fixation, and process durations on cardiovascular tissue imaging with second harmonic generation and multi-photon modalities.Frontiers in bioengineering and biotechnology · 2025Article
- Counteractive effects of extracts ofHeliyon · 2023Article
- Deep learning-based adaptive optics for light sheet fluorescence microscopy.Biomedical optics express · 2023Article
- Light sheet imaging and interactive analysis of the cardiac structure in neonatal mice.Journal of biophotonics · 2023Article
- Review
- Quantitative analysis of illumination and detection corrections in adaptive light sheet fluorescence microscopy.Biomedical optics express · 2022Article
- Tissue clearing to examine tumour complexity in three dimensions.Nature reviews. Cancer · 2021Review
- Review
- Tissue Optical Clearing for Biomedical Imaging: From In Vitro to In Vivo.Advances in experimental medicine and biology · 2021Article
- Three-dimensional Imaging Coupled with Topological Quantification Uncovers Retinal Vascular Plexuses Undergoing Obliteration.Theranostics · 2021Article
- Biomedical Applications of Tissue Clearing and Three-Dimensional Imaging in Health and Disease.iScience · 2020Review
- Clearing techniques for visualizing the nervous system in development, injury, and disease.Journal of neuroscience methods · 2020Review
- A cationic near infrared fluorescent agent and ethyl-cinnamate tissue clearing protocol for vascular staining and imaging.Scientific reports · 2019Article
- Advanced microscopy to elucidate cardiovascular injury and regeneration: 4D light-sheet imaging.Progress in biophysics and molecular biology · 2018Review
- Light-sheet Fluorescence Microscopy for the Study of the Murine Heart.Journal of visualized experiments : JoVE · 2018Article
- Advances in CLARITY-based tissue clearing and imaging.Experimental and therapeutic medicine · 2018Review
- Multiscale light-sheet for rapid imaging of cardiopulmonary system.JCI insight · 2018Review
- Whole-body clearing, staining and screening of calcium deposits in the mdx mouse model of Duchenne muscular dystrophy.Skeletal muscle · 2018Article
- Topological Arrangement of Cardiac Fibroblasts Regulates Cellular Plasticity.Circulation research · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors.
Funding
Abstract
Tissue clearing methods promise to provide exquisite three-dimensional imaging information; however, there is a need for simplified methods for lower resource settings and for non-fluorescence based phenotyping to enable light microscopic imaging modalities. Here we describe the simplified CLARITY method (SCM) for tissue clearing that preserves epitopes of interest. We imaged the resulting tissues using light sheet microscopy to generate rapid 3D reconstructions of entire tissues and organs. In addition, to enable clearing and 3D tissue imaging with light microscopy methods, we developed a colorimetric, non-fluorescent method for specifically labeling cleared tissues based on horseradish peroxidase conversion of diaminobenzidine to a colored insoluble product. The methods we describe here are portable and can be accomplished at low cost, and can allow light microscopic imaging of cleared tissues, thus enabling tissue clearing and imaging in a wide variety of settings.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.