Evidence map›Paper›PMID 27498216›Full record

ArticleCryobiology2016

Cryopreservation of human spermatozoa with minimal non-permeable cryoprotectant.

Jie Liu, Cigdem Tanrikut, Diane L Wright, Gloria Y Lee, Mehmet Toner, John D Biggers, Thomas L Toth

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cryobiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
5.3field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 43 citations in OpenAlex.

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  15. Cryopreservation and its clinical applications.Integrative medicine research · 2017
    Review
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Jie LiuDepartment of Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA; Shriners Hospital for Children, Boston, MA 02114, USA.
Cigdem TanrikutDepartment of Urology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.
Diane L WrightDepartment of Obstetrics and Gynecology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.
Gloria Y LeeDepartment of Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA; Shriners Hospital for Children, Boston, MA 02114, USA.
Mehmet TonerDepartment of Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA; Shriners Hospital for Children, Boston, MA 02114, USA.
John D BiggersDepartment of Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA; Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA; Shriners Hospital for Children, Boston, MA 02114, USA. Electronic address: John_Biggers@hms.harvard.edu.
Thomas L TothDepartment of Obstetrics and Gynecology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA. Electronic address: TLTOTH@mgh.harvard.edu.
Massachusetts General Hospital · USHarvard University · US

Funding

Bio-MicroElectroMechanical Systems Resource CenterP41EB002503 · NIBIB · MASSACHUSETTS GENERAL HOSPITAL · PI TONER, MEHMET · 2004 to 2018
$17.4M
Simple and Cost-effective Storage of Mouse Sperm using Evaporative DryingR24OD016985 · OD · MASSACHUSETTS GENERAL HOSPITAL · PI BIGGERS, JOHN D, TONER, MEHMET · 2014 to 2017
$2.5M
Novel Microcapillary Technology for the Cryopreservation of Human OocytesR21HD061297 · NICHD · MASSACHUSETTS GENERAL HOSPITAL · PI TONER, MEHMET · 2009 to 2010
$484k
NIBIB NIH HHS P41 EB002503NICHD NIH HHS R21 HD061297NIH HHS R24 OD016985
6 · The paper itself

Abstract

Cryopreservation of human spermatozoa is a commonly used technique in assisted reproduction, however freezing low concentrations of sperm while maintaining adequate post-thaw motility remains a challenge. In an effort to optimize post-thaw motility yields, low volumes of human sperm were frozen in polyimide-coated fused silica micro-capillaries using 0.065 M, 0.125 M, 0.25 M, or 0.5 M trehalose as the only cryoprotectant. Micro-capillaries were either initially incubated in liquid nitrogen vapor before plunging into liquid nitrogen, or directly plunged into liquid nitrogen. Post thaw sperm counts and motility were estimated. Spermatozoa that were initially incubated in liquid nitrogen vapor had greater post thaw motility than those plunged immediately into liquid nitrogen independent of trehalose concentration. The protective effect of 0.125 M d-glucose, 3-O-methyl-d-glucopyranose, trehalose, sucrose, raffinose, or stachyose were evaluated individually. Trehalose and sucrose were the most effective cryoprotectants, recovering 69.0% and 68.9% of initial sperm motility, respectively.

Indexed as

3-O-MethylglucoseAnimalsCryopreservationCryoprotective AgentsFreezingGlucoseHumansMaleOligosaccharidesRaffinoseSemen PreservationSpermatozoaSperm MotilitySucroseTrehalose3-O-MethylglucoseCryoprotective AgentsGlucoseOligosaccharidesRaffinosestachyoseSucroseTrehaloseCryoprotectantSilica micro-capillarySperm cryopreservationSugars

Identifiers

PMID27498216
PMCPMC5519348
OpenAlexW2483269429

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.