Evidence map›Paper›PMID 27497818›Full record

ArticleMolecular biology reports2016

Nucleotide variation of sFRP5 gene is not associated with obesity in children and adolescents.

Jasmijn K Van Camp, Sigri Beckers, Doreen Zegers, Stijn L Verhulst, Kim Van Hoorenbeeck, Guy Massa, An Verrijken, Kristine N Desager, Luc F Van Gaal, Wim Van Hul

Abstract read
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In one paragraph

Article in Molecular biology reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Review
  2. Sfrp5/Wnt Pathway: A Protective Regulatory System in Atherosclerotic Cardiovascular Disease.Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research · 2019
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Jasmijn K Van CampDepartment of Medical Genetics, University of Antwerp, Universiteitsplein 1, 2610, Antwerp, Belgium.
Sigri BeckersDepartment of Medical Genetics, University of Antwerp, Universiteitsplein 1, 2610, Antwerp, Belgium.
Doreen ZegersDepartment of Medical Genetics, University of Antwerp, Universiteitsplein 1, 2610, Antwerp, Belgium.
Stijn L VerhulstDepartment of Paediatrics, Antwerp University Hospital, Wilrijkstraat 10, 2650, Antwerp, Belgium.
Kim Van HoorenbeeckDepartment of Paediatrics, Antwerp University Hospital, Wilrijkstraat 10, 2650, Antwerp, Belgium.
Guy MassaDepartment of Paediatrics, Jessa Hospital, Stadsomvaart 11, 3500, Hasselt, Belgium.
An VerrijkenDepartment of Endocrinology, Diabetology and Metabolic Diseases, Antwerp University Hospital, Wilrijkstraat 10, 2650, Antwerp, Belgium.
Kristine N DesagerDepartment of Paediatrics, Antwerp University Hospital, Wilrijkstraat 10, 2650, Antwerp, Belgium.
Luc F Van GaalDepartment of Endocrinology, Diabetology and Metabolic Diseases, Antwerp University Hospital, Wilrijkstraat 10, 2650, Antwerp, Belgium.
Wim Van HulDepartment of Medical Genetics, University of Antwerp, Universiteitsplein 1, 2610, Antwerp, Belgium. wim.vanhul@uantwerpen.be.
Antwerp University Hospital · BEUniversity of Antwerp · BEJessa Hospital · BE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Because sFRP5 was shown to be an important extracellular modulator of the Wnt pathway, regulating adipogenesis, we wanted to investigate the role of sFRP5 variants in human, monogenic obesity by performing mutation analysis. We screened the complete sFRP5 coding region in 622 obese children and adolescents and 503 lean control individuals by high-resolution melting curve analysis and direct sequencing. We found a total of 15 sequence variants in sFRP5, 10 of which resulted in a non-synonymous amino acid change. Five of these variants were, to our knowledge, not previously reported. For one of the variants (c.-3G>A), we identified a trend towards association between the variant frequency and the obese phenotype. We argue that, when looking at conservation and location inside known protein domains, several of the identified variants (D103N, A113V, K212N and H317L), may affect sFRP5 protein function. In addition, we found c.-3G>A, residing in the Kozak sequence, with a lower frequency in cases compared to controls. However, functional studies investigating the effect of sFRP5 variants on protein function are necessary to determine the true role of sFRP5 genetic variation in human, monogenic obesity.

Indexed as

Polymorphism, Single NucleotideAdaptor Proteins, Signal TransducingAdolescentChildEye ProteinsFemaleGene FrequencyGenetic Association StudiesHumansMaleMembrane ProteinsNucleotidesObesitySecreted Frizzled-Related ProteinsSequence Analysis, DNAAdaptor Proteins, Signal TransducingEye ProteinsMembrane ProteinsNucleotidesSecreted Frizzled-Related ProteinsSFRP5 protein, humanGeneticsMutation analysisObesitysFRPWnt

Identifiers

PMID27497818
OpenAlexW2504990927

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.