ArticleGenome biology and evolution2016
Centromeres of the Yeast Komagataella phaffii (Pichia pastoris) Have a Simple Inverted-Repeat Structure.
Article in Genome biology and evolution, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 41 citations in OpenAlex.
- Article
- Centromeres in budding yeasts are conserved in chromosomal location but not in structure.PLoS genetics · 2025Article
- Centromeric Sequences in Ogataea polymorpha Genome Enable Development of Stable Multigene Expression Plasmid Tools.Microbial biotechnology · 2025Article
- Decoding the Genome of the Komagataella phaffii Type Strain CBS 2612.Yeast (Chichester, England) · 2025Article
- What makes Komagataella phaffii non-conventional?FEMS yeast research · 2021Review
- The Curious Case of Nonrepetitive Centromeric DNA Sequences in Candida auris and Related Species.mBio · 2021Article
- Recombination machinery engineering facilitates metabolic engineering of the industrial yeast Pichia pastoris.Nucleic acids research · 2021Article
- Article
- Spatial inter-centromeric interactions facilitated the emergence of evolutionary new centromeres.eLife · 2020Article
- Polymorphic centromere locations in the pathogenic yeastGenome research · 2020Article
- Construction and characterization of centromeric plasmids for Komagataella phaffii using a color-based plasmid stability assay.PloS one · 2020Article
- Review
- Genomic diversity and meiotic recombination among isolates of the biotech yeast Komagataella phaffii (Pichia pastoris).Microbial cell factories · 2019Article
- The Methylotroph Gene Order Browser (MGOB) reveals conserved synteny and ancestral centromere locations in the yeast family Pichiaceae.FEMS yeast research · 2019Article
- Multiple Reinventions of Mating-type Switching during Budding Yeast Evolution.Current biology : CB · 2019Article
- Discovery and Evolution of New Domains in Yeast Heterochromatin Factor Sir4 and Its Partner Esc1.Genome biology and evolution · 2019Article
- A Stable, Autonomously Replicating Plasmid Vector Containing Pichia pastoris Centromeric DNA.Applied and environmental microbiology · 2018Article
- Population genomics shows no distinction between pathogenic Candida krusei and environmental Pichia kudriavzevii: One species, four names.PLoS pathogens · 2018Article
- Creation of Stable Heterothallic Strains of Komagataella phaffii Enables Dissection of Mating Gene Regulation.Molecular and cellular biology · 2018Article
- Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Centromere organization has evolved dramatically in one clade of fungi, the Saccharomycotina. These yeasts have lost the ability to make normal eukaryotic heterochromatin with histone H3K9 methylation, which is a major component of pericentromeric regions in other eukaryotes. Following this loss, several different types of centromere emerged, including two types of sequence-defined ("point") centromeres, and the epigenetically defined "small regional" centromeres of Candida albicans Here we report that centromeres of the methylotrophic yeast Komagataella phaffii (formerly called Pichia pastoris) are structurally defined. Each of its four centromeres consists of a 2-kb inverted repeat (IR) flanking a 1-kb central core (mid) region. The four centromeres are unrelated in sequence. CenH3 (Cse4) binds strongly to the cores, with a decreasing gradient along the IRs. This mode of organization resembles Schizosaccharomyces pombe centromeres but is much more compact and lacks the extensive flanking heterochromatic otr repeats. Different isolates of K. phaffii show polymorphism for the orientation of the mid regions, due to recombination in the IRs. CEN4 is located within a 138-kb region that changes orientation during mating-type switching, but switching does not induce recombination of centromeric IRs. Our results demonstrate that evolutionary transitions in centromere organization have occurred in multiple yeast clades.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.