ArticleNucleic acids research2016
Efficiency of integron cassette insertion in correct orientation is ensured by the interplay of the three unpaired features of attC recombination sites.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 49 citations in OpenAlex.
- High-throughput recovery of integron cassettes for gene discovery screens.Nature microbiology · 2026Article
- Identification and characteristics of a novel acquired aminoglycoside phosphotransferase, APH(3')-IVb, fromAntimicrobial agents and chemotherapy · 2026Article
- OptimDase: An Algorithm for Predicting DNA Binding Sites with Combined Feature Encoding.Interdisciplinary sciences, computational life sciences · 2025Article
- Bacterial natural transformation drives cassette shuffling and simplifies recombination in chromosomal integrons.Nucleic acids research · 2025Article
- Integrons are anti-phage defence libraries in Vibrio parahaemolyticus.Nature microbiology · 2025Article
- The recombination efficiency of the bacterial integron depends on the mechanical stability of the synaptic complex.Science advances · 2024Article
- Belt and braces: Two escape ways to maintain the cassette reservoir of large chromosomal integrons.PLoS genetics · 2024Article
- Cassette recombination dynamics within chromosomal integrons are regulated by toxin-antitoxin systems.Science advances · 2024Article
- Integron cassettes integrate into bacterial genomes via widespread non-classical attG sites.Nature microbiology · 2024Article
- Functional enrichment of integrons: Facilitators of antimicrobial resistance and niche adaptation.iScience · 2023Article
- Influence of class 2 integron integrase concentration on gene cassette insertion and excision in vivo.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Sub-inhibitory gentamicin pollution induces gentamicin resistance gene integration in class 1 integrons in the environment.Scientific reports · 2023Article
- Article
- Identification of integrons and gene cassette-associated recombination sites in bacteriophage genomes.Frontiers in microbiology · 2023Article
- Discovery of integrons in Archaea: Platforms for cross-domain gene transfer.Science advances · 2022Article
- Article
- Unbridled Integrons: A Matter of Host Factors.Cells · 2022Review
- Methods for the targeted sequencing and analysis of integrons and their gene cassettes from complex microbial communities.Microbial genomics · 2022Article
- Integron Functionality and Genome Innovation: An Update on the Subtle and Smart Strategy of Integrase and Gene Cassette Expression Regulation.Microorganisms · 2022Review
- Predicting the taxonomic and environmental sources of integron gene cassettes using structural and sequence homology of attC sites.Communications biology · 2021Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The integron is a bacterial recombination system that allows acquisition, stockpiling and expression of cassettes carrying protein-coding sequences, and is responsible for the emergence and rise of multiresistance in Gram-negative bacteria. The functionality of this system depends on the insertion of promoterless cassettes in correct orientation, allowing their expression from the promoter located upstream of the cassette array. Correct orientation is ensured by strand selectivity of integron integrases for the bottom strand of cassette recombination sites (attC), recombined in form of folded single-stranded hairpins. Here, we investigated the basis of such strand selectivity by comparing recombination of wild-type and mutated attC sites with different lengths, sequences and structures. We show that all three unpaired structural features that distinguish the bottom and top strands contribute to strand selectivity. The localization of Extra-Helical Bases (EHBs) directly favors integrase binding to the bottom strand. The Unpaired Central Spacer (UCS) and the Variable Terminal Structure (VTS) influence strand selectivity indirectly, probably through the stabilization of the bottom strand and the resulting synapse due to the nucleotide skew between the two strands. These results underscore the importance of the single-stranded nature of the attC site that allows such tight control over integron cassette orientation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.