ArticleMalaria journal2016
Diagnosis and treatment based on quantitative PCR after controlled human malaria infection.
Article in Malaria journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 41 citations in OpenAlex.
- Trial
- Trial
- Controlled Human Malaria Infection of Healthy Adults With Lifelong Malaria Exposure to Assess Safety, Immunogenicity, and Efficacy of the Asexual Blood Stage Malaria Vaccine Candidate GMZ2.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2019Trial
- Outcomes of controlled human malaria infection after BCG vaccination.Nature communications · 2019Trial
- Controlled Human Malaria Infection with Graded Numbers ofThe American journal of tropical medicine and hygiene · 2018Trial
- Modest heterologous protection after Plasmodium falciparum sporozoite immunization: a double-blind randomized controlled clinical trial.BMC medicine · 2017Trial
- Diagnosis of Plasmodium falciparum malaria at very low parasitaemias using a commercially available LAMP assay and RDT.Transactions of the Royal Society of Tropical Medicine and Hygiene · 2025Article
- Article
- Symptoms and adverse events in controlled human infection models.Frontiers in medicine · 2025Review
- Breath Biomarkers of Pediatric Malaria: Reproducibility and Response to Antimalarial Therapy.The Journal of infectious diseases · 2024Article
- Plasmodium falciparum Malaria Is Associated With Increased Kaposi Sarcoma-Associated Herpesvirus (KSHV) Seropositivity and Higher KSHV Antibody Breadth and Magnitude: Results of a Case-Control Study From Rural Uganda.The Journal of infectious diseases · 2024Article
- Analytical validation of a real-time hydrolysis probe PCR assay for quantifying Plasmodium falciparum parasites in experimentally infected human adults.Malaria journal · 2021Article
- Recombinase Polymerase Amplification and Lateral Flow Assay for Ultrasensitive Detection of Low-Density Plasmodium falciparum Infection from Controlled Human Malaria Infection Studies and Naturally Acquired Infections.Journal of clinical microbiology · 2020Article
- Imported Malaria in Countries where Malaria Is Not Endemic: a Comparison of Semi-immune and Nonimmune Travelers.Clinical microbiology reviews · 2020Review
- A controlled human Schistosoma mansoni infection model to advance novel drugs, vaccines and diagnostics.Nature medicine · 2020Article
- Controlled Human Malaria Infection Induces Long-Term Functional Changes in Monocytes.Frontiers in molecular biosciences · 2020Article
- Fast and fierce versus slow and smooth: Heterogeneity in immune responses to Plasmodium in the controlled human malaria infection model.Immunological reviews · 2020Review
- Signatures of divergent anti-malarial treatment responses in peripheral blood from adults and young children in Malawi.Malaria journal · 2019Article
- The Complement System Contributes to Functional Antibody-Mediated Responses Induced by Immunization with Plasmodium falciparum Malaria Sporozoites.Infection and immunity · 2018Article
- Mosquito Infectivity and Parasitemia after Controlled Human Malaria Infection.The American journal of tropical medicine and hygiene · 2018Article
Corrections and comments
- Erratum issued
Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundControlled human malaria infection (CHMI) has become well-established in the evaluation of drugs and vaccines. Anti-malarial treatment is usually initiated when thick blood smears are positive by microscopy. This study explores the effects of using the more sensitive qPCR as the primary diagnostic test.
methods1691 diagnostic blood samples were analysed by microscopy and qPCR from 115 volunteers (55 malaria naïve and 60 having received chemoprophylaxis and sporozoite immunization) who were challenged by five mosquitoes infected with Plasmodium falciparum sporozoites of the NF54 strain.
resultsRetrospective analysis of different qPCR criteria for diagnosis and treatment, showed that once daily qPCR (threshold 100 parasites/ml) had 99 % sensitivity and 100 % specificity, and shortened the median prepatent period from 10.5 to 7.0 days after CHMI when compared to twice daily measurement of thick blood smears (threshold 4000 parasites/ml). This is expected to result in a 78 % decrease of adverse events before initiation of treatment in future studies. Trial outcome related to infection and protective efficacy remained unchanged.
conclusionThe use of qPCR as the primary diagnostic test in CHMI decreases symptoms as well as parasitaemia while obviating the need for twice daily follow-up. The implementation improves safety while reducing the clinical burden and costs without compromising the evaluation of protective efficacy.
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