ArticleJournal of neuroscience methods2016
A system to measure the pupil response to steady lights in freely behaving mice.
Article in Journal of neuroscience methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 14 citations in OpenAlex.
- A transparent wheel-based platform for locomotion-on-demand and multi-view body and facial kinematics in head-fixed mice.Scientific reports · 2026Article
- Retinal Cx36 gap junctions in the inner and outer plexiform layers differentially control visual thresholds.iScience · 2026Article
- Mitochondrial protein import stress augments α-synuclein aggregation and neural damage independent of bioenergetics.Research square · 2025Article
- In Vivo Quantification of Anterior and Posterior Chamber Volumes in Mice: Implications for Aqueous Humor Dynamics.Investigative ophthalmology & visual science · 2025Article
- Signal Detection Theoretic Estimates of the Murine Absolute Visual Threshold Are Independent of Decision Bias.eNeuro · 2024Article
- An Easily Compatible Eye-tracking System for Freely-moving Small Animals.Neuroscience bulletin · 2022Article
- Cone-Driven Retinal Responses Are Shaped by Rod But Not Cone HCN1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Night skies through animals' eyes-Quantifying night-time visual scenes and light pollution as viewed by animals.Frontiers in cellular neuroscience · 2022Article
- Acepromazine and Chlorpromazine as Pharmaceutical-grade Alternatives to Chlorprothixene for Pupillary Light Reflex Imaging in Mice.Journal of the American Association for Laboratory Animal Science : JAALAS · 2020Article
- Rod Photoreceptors Signal Fast Changes in Daylight Levels Using a Cx36-Independent Retinal Pathway in Mouse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Rod Photoresponse Kinetics Limit Temporal Contrast Sensitivity in Mesopic Vision.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- High-Throughput Automatic Training System for Odor-Based Learned Behaviors in Head-Fixed Mice.Frontiers in neural circuits · 2018Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundTransgenic mice are widely used for the study of basic visual function and retinal disease, including in psychophysical tests. Mice have a robust pupillary light reflex that controls the amount of light that enters the eye, and the attenuating effects of the pupil must be considered during such tests. Measurement of the size of pupils at various luminance levels requires that mice remain stable over prolonged periods of time; however, sedation of mice with anesthesia and/or manual restraint can influence the size of their pupils. NEW
methodWe present a system to measure the pupillary light response to steady lights of freely behaving mice using a custom-built, portable device that automatically acquires close-up images of their eyes. The device takes advantage of the intrinsic nature of mice to inspect objects of interest and can be used to measure pupillary responses in optomotor or operant behavior testing chambers.
resultsThe size of the pupils in freely behaving mice decreased gradually with luminance from a maximal area in the dark of 3.8mm COMPARISON WITH EXISTING
methodsThese values agree with prior measurements of the pupillary response of unrestrained mice that use more laborious and time consuming approaches.
conclusionsOur new method facilitates practical, straightforward and accurate measurements of pupillary responses made under the same experimental conditions as those used during psychophysical testing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.