ArticlePloS one2016
Circadian Rhythm Regulates Development of Enamel in Mouse Mandibular First Molar.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- Melatonin Rescues Enamel Defects Induced by Maternal Circadian Disruption via Targeting the BMAL1-JNK3 Axis.International dental journal · 2026Article
- p75NTR Promotes Circadian-Driven Mineralization During Tooth Development via CK2/PER2 Pathway.Cell proliferation · 2026Article
- Circadian rhythm disruption impairs time-dependent dentine regeneration and reprograms the late-stage dental proteome in a mouse pulp injury model.Frontiers in pharmacology · 2026Article
- The role of the TGF-β1 signaling pathway in the process of amelogenesis.Frontiers in physiology · 2025Review
- Melatonin ameliorates inflammation-induced developmental defects of enamel by upregulating regulator of G protein signaling 2.Journal of dental sciences · 2024Article
- The circadian clock in enamel development.International journal of oral science · 2024Review
- Article
- The contribution of circadian clock to the biological processes.Frontiers in molecular biosciences · 2024Review
- Absence of melatonin during development impairs craniofacial and dental onset in rats.Clinical oral investigations · 2023Article
- Neural Regulations in Tooth Development and Tooth-Periodontium Complex Homeostasis: A Literature Review.International journal of molecular sciences · 2022Review
- Article
- The effect of melatonin on the mouse ameloblast-lineage cell line ALCs.Scientific reports · 2022Article
- A potential role ofFrontiers in physiology · 2022Article
- Circadian clock-A promising scientific target in oral science.Frontiers in physiology · 2022Review
- Melatonin-Medicated Neural JNK3 Up-Regulation Promotes Ameloblastic Mineralization.Frontiers in cell and developmental biology · 2021Article
- Biological clocks and incremental growth line formation in dentine.Journal of anatomy · 2020Article
- Chronodentistry: the role & potential of molecular clocks in oral medicine.BMC oral health · 2019Review
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rhythmic incremental growth lines and the presence of melatonin receptors were discovered in tooth enamel, suggesting possible role of circadian rhythm. We therefore hypothesized that circadian rhythm may regulate enamel formation through melatonin receptors. To test this hypothesis, we examined expression of melatonin receptors (MTs) and amelogenin (AMELX), a maker of enamel formation, during tooth germ development in mouse. Using qRT-PCR and immunocytochemistry, we found that mRNA and protein levels of both MTs and AMELX in normal mandibular first molar tooth germs increased gradually after birth, peaked at 3 or 4 day postnatal, and then decreased. Expression of MTs and AMELX by immunocytochemistry was significantly delayed in neonatal mice raised in all-dark or all-light environment as well as the enamel development. Furthermore, development of tooth enamel was also delayed showing significant immature histology in those animals, especially for newborn mice raised in all daylight condition. Interestingly, disruption in circadian rhythm in pregnant mice also resulted in delayed enamel development in their babies. Treatment with melatonin receptor antagonist 4P-PDOT in pregnant mice caused underexpression of MTs and AMELX associated with long-lasting deficiency in baby enamel tissue. Electromicroscopic evidence demonstrated increased necrosis and poor enamel mineralization in ameloblasts. The above results suggest that circadian rhythm is important for normal enamel development at both pre- and postnatal stages. Melatonin receptors were partly responsible for the regulation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.