ArticleJournal of cachexia, sarcopenia and muscle2016
p62/SQSTM1 but not LC3 is accumulated in sarcopenic muscle of mice.
Article in Journal of cachexia, sarcopenia and muscle, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed.
- Protein arginine methyltransferases coordinate mitochondrial stress adaptation and neuromuscular function.Experimental & molecular medicine · 2026Review
- Loss of Atoh8 Impairs Macroautophagy.Cells · 2025Article
- The ubiquitin-conjugating enzyme UBE2D maintains a youthful proteome and ensures protein quality control during aging by sustaining proteasome activity.PLoS biology · 2025Article
- Extra-osseous Roles of the RANK-RANKL-OPG Axis with a Focus on Skeletal Muscle.Current osteoporosis reports · 2024Review
- Age-related histone H3.3 accumulation associates with a repressive chromatin in mouse tibialis anterior muscle.The journal of physiological sciences : JPS · 2024Article
- Life-Long Aerobic Exercise is a Non-Pharmacological Approach for Inducing Autophagy and Delaying Muscle Atrophy in the Aging Population.Aging and disease · 2024Review
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- Lysosomes as coordinators of cellular catabolism, metabolic signalling and organ physiology.Nature reviews. Molecular cell biology · 2024Review
- Obesity worsens mitochondrial quality control and does not protect against skeletal muscle wasting in murine cancer cachexia.Journal of cachexia, sarcopenia and muscle · 2024Article
- Review
- Autophagy signaling in hypertrophied muscles of diabetic and control rats.FEBS open bio · 2023Article
- Age-Related Dysfunction in Proteostasis and Cellular Quality Control in the Development of Sarcopenia.Cells · 2023Review
- PD-1 Alleviates Cisplatin-Induced Muscle Atrophy by Regulating Inflammation and Oxidative Stress.Antioxidants (Basel, Switzerland) · 2022Article
- Role of mitophagy in the hallmarks of aging.Journal of biomedical research · 2022Article
- p62/Sqstm1 rescue in muscle retards the progression of steatohepatitis inFrontiers in physiology · 2022Article
- Integrated genomic and proteomic analyses identify stimulus-dependent molecular changes associated with distinct modes of skeletal muscle atrophy.Cell reports · 2021Article
- Analysis of proteostasis during aging with western blot of detergent-soluble and insoluble protein fractions.STAR protocols · 2021Article
- miR-130b inhibits proliferation and promotes differentiation in myocytes via targeting Sp1.Journal of molecular cell biology · 2021Article
- Mitochondrial Dynamics and Mitophagy in Skeletal Muscle Health and Aging.International journal of molecular sciences · 2021Review
- The emerging role of the sympathetic nervous system in skeletal muscle motor innervation and sarcopenia.Ageing research reviews · 2021Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimWe investigated the pathway of autophagy signaling linked to sarcopenia of mice.
methodsYoung adult (3-month) and aged (24- month) C57BL/6J mice were used. Using real-time PCR, Western blotting, and immunohistochemical microscopy, we evaluated the amounts of p62/SQSTM1, LC3, and Beclin-1 in the quadriceps muscle change with aging in mice.
resultsMarked fiber atrophy (30%) and many fibers with central nuclei were observed in the aged mice. Western blotting using homogenate of the cytosolic fraction clearly showed that the amounts of p62/SQSTM1 and Beclin-1 proteins were significantly increased in the aged skeletal muscle. The amounts of these proteins in both nuclear and membrane fractions did not change significantly with age. Immunofluorescence labeling indicated that aged mice more frequently possessed p62/SQSTM1-positive fibers in the cytosol in quadriceps muscle than young ones (aged: 14% vs. young: 1%). In aged muscle, p62/SQSTM1-positive fibers were significantly smaller than the surrounding p62/SQSTM1-negative fibers. Aging did not elicit significant changes in the mRNA levels of p62/SQSTM1 and Beclin-1, but decreased LC3 mRNA level. In aged muscle, the location of p62/SQSTM1 immunoreactivity was similar to that of Beclin-1 protein, but not LC3.
conclusionSarcopenia in mice appears to include a marked defect of autophagy signaling.
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