ArticleBiophysics and physicobiology2015
Subattomole detection of adiponectin in urine by ultrasensitive ELISA coupled with thio-NAD cycling.
Article in Biophysics and physicobiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 19 citations in OpenAlex.
- Comparison of urinary adiponectin in the presence of metabolic syndrome in peri- and postmenopausal women.BMC women's health · 2022Article
- Modified ELISA for Ultrasensitive Diagnosis.Journal of clinical medicine · 2021Review
- Zeptomole Detection of an Enzyme by a Simple Colorimetric Method.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2021Article
- Antigen tests for COVID-19.Biophysics and physicobiology · 2021Article
- A novel, rapid (within hours) culture-free diagnostic method for detecting live Mycobacterium tuberculosis with high sensitivity.EBioMedicine · 2020Article
- Proposal of De Novo Antigen Test for COVID-19: Ultrasensitive Detection of Spike Proteins of SARS-CoV-2.Diagnostics (Basel, Switzerland) · 2020Article
- Ultrasensitive ELISA Developed for Diagnosis.Diagnostics (Basel, Switzerland) · 2019Review
- A novel role of oxytocin: Oxytocin-induced well-being in humans.Biophysics and physicobiology · 2019Article
- Urinary adiponectin as a new diagnostic index for chronic kidney disease due to diabetic nephropathy.BMJ open diabetes research & care · 2019Article
- Ultrasensitive detection of proteins and sugars at single-cell level.Communicative & integrative biologyReview
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adiponectin is a hormone secreted from adipocytes, and it demonstrates antidiabetic, anti-atherosclerotic, antiobesity and anti-inflammatory effects. However, the patterns of change in urinary adiponectin levels in various diseases remain unknown, because only trace amounts of the hormone are present in urine. In the present study, we applied an ultrasensitive ELISA coupled with thio-NAD cycling to measure urinary adiponectin levels. Spikeand-recovery tests using urine confirmed the reliability of our ultrasensitive ELISA. The limit of detection for adiponectin in urine was 2.3×10(-19) moles/assay (1.4 pg/mL). The urinary adiponectin concentration ranged between 0.04 and 5.82 ng/mL in healthy subjects. The pilot study showed that the urinary adiponectin levels, which were corrected by the creatinine concentration, were 0.73±0.50 (ng/mg creatinine, N=6) for healthy subjects, versus 12.02±3.85 (ng/mg creatinine, N=3) for patients with diabetes mellitus (DM). That is, the urinary adiponectin levels were higher (P<0.05) in DM patients than in healthy subjects. Further, these urinary adiponectin levels tended to increase with the progression of DM accompanied with nephropathy. Our method is thus expected to provide a simple, rapid and reasonably priced test for noninvasive monitoring of the progression of DM without the requirement of special tools.
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