ArticleFrontiers in plant science2016
Geminivirus-Mediated Genome Editing in Potato (Solanum tuberosum L.) Using Sequence-Specific Nucleases.
Article in Frontiers in plant science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 125 papers.
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Who cites it
125 citing papers in PubMed, 334 citations in OpenAlex.
- Isoform-specific function underlies differential herbicide resistance of the W574L mutation in peanut AhALS isozymes.Pest management science · 2026Article
- From Lab to Field: CRISPRing Major CultivatedInternational journal of molecular sciences · 2026Review
- Development of Modular Geminivirus-Based Vectors for High Cargo Expression and Gene Targeting in Plants.Plant biotechnology journal · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Engineering Conditional Transgene Expression in Nicotiana benthamiana.Plant biotechnology journal · 2026Review
- Geminivirus-derived replicons: assessment for transient GFP expression in tobacco and tomato.Frontiers in plant science · 2026Article
- CRISPR-Cas technologies for precision genome editing in plants: advances, applications, and future perspectives.Frontiers in plant science · 2026Review
- A CRISPR/Cas9-induced restoration of bioluminescence reporter system for single-cell gene expression analysis in plants.Scientific reports · 2025Article
- CRISPR-Mediated Genome Editing in Peanuts: Unlocking Trait Improvement for a Sustainable Future.Plants (Basel, Switzerland) · 2025Review
- Bioluminescence-Driven Optimization of Geminivirus-Based Vectors as Tools for Plant Biotechnology.ACS synthetic biology · 2025Article
- CRISPR-Cas Gene Editing Technology in Potato.International journal of molecular sciences · 2025Review
- CRISPR/Cas genome editing in soybean: challenges and new insights to overcome existing bottlenecks.Journal of advanced research · 2025Review
- Virus-Induced Genome Editing (VIGE): One Step Away from an Agricultural Revolution.International journal of molecular sciences · 2025Review
- Review
- The Application of DNA Viruses to Biotechnology.Viruses · 2025Review
- Beyond a few bases: methods for large DNA insertion and gene targeting in plants.The Plant journal : for cell and molecular biology · 2025Review
- A Gemini Virus-Derived Autonomously Replicating System for HDR-Mediated Genome Editing of thePlants (Basel, Switzerland) · 2025Article
- Advancing vegetable genetics with gene editing: a pathway to food security and nutritional resilience in climate-shifted environments.Functional & integrative genomics · 2025Review
- Advances in genome editing in plants within an evolving regulatory landscape, with a focus on its application in wheat breeding.Journal of plant biochemistry and biotechnology · 2025Review
- Single-stranded DNA (ssDNA) donor repair templates and CRISPR/Cas9 enable a high-frequency of targeted insertions in potato.Frontiers in genome editing · 2025Article
65 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genome editing using sequence-specific nucleases (SSNs) is rapidly being developed for genetic engineering in crop species. The utilization of zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats/CRISPR-associated systems (CRISPR/Cas) for inducing double-strand breaks facilitates targeting of virtually any sequence for modification. Targeted mutagenesis via non-homologous end-joining (NHEJ) has been demonstrated extensively as being the preferred DNA repair pathway in plants. However, gene targeting via homologous recombination (HR) remains more elusive but could be a powerful tool for directed DNA repair. To overcome barriers associated with gene targeting, a geminivirus replicon (GVR) was used to deliver SSNs targeting the potato ACETOLACTATE SYNTHASE1 (ALS1) gene and repair templates designed to incorporate herbicide-inhibiting point mutations within the ALS1 locus. Transformed events modified with GVRs held point mutations that were capable of supporting a reduced herbicide susceptibility phenotype, while events transformed with conventional T-DNAs held no detectable mutations and were similar to wild-type. Regeneration of transformed events improved detection of point mutations that supported a stronger reduced herbicide susceptibility phenotype. These results demonstrate the use of geminiviruses for delivering genome editing reagents in plant species, and a novel approach to gene targeting in a vegetatively propagated species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.