ArticleScientific reports2016
Simulated Microgravity and 3D Culture Enhance Induction, Viability, Proliferation and Differentiation of Cardiac Progenitors from Human Pluripotent Stem Cells.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
What it found
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Who cites it
61 citing papers in PubMed, 102 citations in OpenAlex.
- Unconventional bioprinting modalities for advanced tissue biofabrication.Biomaterials · 2026Review
- The Heart in Space: Effects of Microgravity on Different Cell Types and Their Functions in the Cardiovascular System.Biomedicines · 2025Review
- The Effects of Microgravity on the Structure and Function of Cardiomyocytes.Biomolecules · 2025Review
- Spaceflight alters protein levels and gene expression associated with stress response and metabolic characteristics in human cardiac spheroids.Biomaterials · 2025Article
- Enhancing human pluripotent stem cell differentiation to cardiomyocytes through cardiac progenitor reseeding and cryopreservation.iScience · 2025Article
- Multiscale and recursive unmixing of spatiotemporal rhythms for live-cell and intravital cardiac microscopy.Nature cardiovascular research · 2025Article
- Ethanol exposure during differentiation of human induced pluripotent stem cells reduces cardiomyocyte generation and alters metabolism.Life sciences · 2025Article
- Human induced pluripotent stem cell-derived cardiomyocytes for disease modeling and drug discovery.Frontiers in bioengineering and biotechnology · 2025Review
- Developing anFrontiers in immunology · 2025Article
- Advancing Cardiac Organoid Engineering Through Application of Biophysical Forces.IEEE reviews in biomedical engineering · 2024Article
- Cellular response in three-dimensional spheroids and tissues exposed to real and simulated microgravity: a narrative review.NPJ microgravity · 2024Review
- Surface tension enables induced pluripotent stem cell culture in commercially available hardware during spaceflight.NPJ microgravity · 2024Article
- Differential Effect of Simulated Microgravity on the Cellular Uptake of Small Molecules.Pharmaceutics · 2024Article
- Discoveries from human stem cell research in space that are relevant to advancing cellular therapies on Earth.NPJ microgravity · 2024Review
- Aging and putative frailty biomarkers are altered by spaceflight.Scientific reports · 2024Article
- High-speed optical imaging with sCMOS pixel reassignment.Nature communications · 2024Article
- Conserved mechanisms of self-renewal and pluripotency in mouse and human ESCs regulated by simulated microgravity using a 3D clinostat.Cell death discovery · 2024Article
- Simulated microgravity improves maturation of cardiomyocytes derived from human induced pluripotent stem cells.Scientific reports · 2024Article
- Organs in orbit: how tissue chip technology benefits from microgravity, a perspective.Frontiers in lab on a chip technologies · 2024Article
- Space microgravity increases expression of genes associated with proliferation and differentiation in human cardiac spheres.NPJ microgravity · 2023Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 4 institutions in 1 country.
Funding
Abstract
Efficient generation of cardiomyocytes from human pluripotent stem cells is critical for their regenerative applications. Microgravity and 3D culture can profoundly modulate cell proliferation and survival. Here, we engineered microscale progenitor cardiac spheres from human pluripotent stem cells and exposed the spheres to simulated microgravity using a random positioning machine for 3 days during their differentiation to cardiomyocytes. This process resulted in the production of highly enriched cardiomyocytes (99% purity) with high viability (90%) and expected functional properties, with a 1.5 to 4-fold higher yield of cardiomyocytes from each undifferentiated stem cell as compared with 3D-standard gravity culture. Increased induction, proliferation and viability of cardiac progenitors as well as up-regulation of genes associated with proliferation and survival at the early stage of differentiation were observed in the 3D culture under simulated microgravity. Therefore, a combination of 3D culture and simulated microgravity can be used to efficiently generate highly enriched cardiomyocytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.