ArticlePlant molecular biology2016
Intron sequences that stimulate gene expression in Arabidopsis.
Article in Plant molecular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 59 citations in OpenAlex.
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- Reduced Cas9 transgene silencing by incorporation of intron sequences.Nature communications · 2025Article
- Genome-Wide Identification and Comprehensive Analysis of Ubiquitin-Specific Protease Gene Family in Soybean (International journal of molecular sciences · 2025Article
- SUPPRESOR OF GAMMA RESPONSE 1 promotes early onset of endoreplication upon DNA double-strand breaks by inducing CCS52A1 expression in Arabidopsis roots.Journal of plant research · 2025Article
- Introns increase gene expression in Caenorhabditis elegans by a mechanism that must be at least partly different than in plants.Scientific reports · 2025Article
- Genome-wide analysis of the KCS gene family in Medicago truncatula and their expression profile under various abiotic stress.Scientific reports · 2025Article
- Expression variation of Viola APETALA3 orthologous genes is correlated with chasmogamous and cleistogamous flower development.BMC plant biology · 2025Article
- Sequence-dependent and -independent effects of intron-mediated enhancement learned from thousands of random introns.Nucleic acids research · 2025Article
- The Intra-Articular Delivery of a Low-Dose Adeno-Associated Virus-IL-1 Receptor Antagonist Vector Alleviates the Progress of Arthritis in an Osteoarthritis Rat Model.Pharmaceutics · 2024Article
- Application of genome editing techniques to regulate gene expression in crops.BMC plant biology · 2024Review
- Intron-mediated enhancement of DIACYLGLYCEROL ACYLTRANSFERASE1 expression in energycane promotes a step change for lipid accumulation in vegetative tissues.Biotechnology for biofuels and bioproducts · 2023Article
- Genome-Wide Identification and Expression Profiling of Potassium Transport-Related Genes inPlants (Basel, Switzerland) · 2021Article
- Identification of cis-regulatory motifs in first introns and the prediction of intron-mediated enhancement of gene expression in Arabidopsis thaliana.BMC genomics · 2021Article
- Genome-wide identification and expression analysis of the AT-hook Motif Nuclear Localized gene family in soybean.BMC genomics · 2021Article
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- Prediction of Rice Transcription Start Sites Using TransPrise: A Novel Machine Learning Approach.Methods in molecular biology (Clifton, N.J.) · 2021Article
- An Intron of Invertebrate Microphthalmia Transcription Factor Gene Is Evolved from a Longer Ancestral Sequence.Evolutionary bioinformatics online · 2021Article
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Authors and funding
4 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
key messageRelated motifs strongly increase gene expression when added to an intron located in coding sequences. Many introns greatly increase gene expression through a mechanism that remains elusive. An obstacle to understanding intron-mediated enhancement (IME) has been the difficulty of locating the specific intron sequences responsible for boosting expression because they are redundant, dispersed, and degenerate. Previously we used the IMEter algorithm in two independent ways to identify two motifs (CGATT and TTNGATYTG) that are candidates for involvement in IME in Arabidopsis. Here we show that both motifs are sufficient to increase expression. An intron that has little influence on expression was converted into one that increased mRNA accumulation 24-fold and reporter enzyme activity 40-fold relative to the intronless control by introducing 11 copies of the more active TTNGATYTG motif. This degree of stimulation is twice as large as that of the strongest of 15 natural introns previously tested in the same reporter gene. Even though the CGATT and TTNGATYTG motifs each increased expression, and CGATT matches the NGATY core of the longer motif, combining the motifs to make TTCGATTTG reduced the stimulating ability of the TTNGATYTG motif. Additional substitutions were used to test the contribution to IME of other residues in the TTNGATYTG motif. The verification that these motifs are active in IME will improve our ability to predict the stimulating ability of introns, to engineer any intron to increase expression to a desired level, and to explore the mechanism of IME by seeking factors that might interact with these sequences.
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