ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2016
The serine protease-mediated increase in intestinal epithelial barrier function is dependent on occludin and requires an intact tight junction.
Article in American journal of physiology. Gastrointestinal and liver physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 29 citations in OpenAlex.
- Proteases in intestinal health and disease.Nature reviews. Gastroenterology & hepatology · 2026Review
- Tmprss2 maintains epithelial barrier integrity and transepithelial sodium transport.Life science alliance · 2024Article
- Article
- Healthy regulation of Tibetan Brassica rapa L. polysaccharides on alleviating hyperlipidemia: A rodent study.Food chemistry. Molecular sciences · 2023Article
- Autophagy Reduces the Degradation and Promotes Membrane Localization of Occludin to Enhance the Intestinal Epithelial Tight Junction Barrier against Paracellular Macromolecule Flux.Journal of Crohn's & colitis · 2023Article
- EpCAM proteolysis and release of complexed claudin-7 repair and maintain the tight junction barrier.The Journal of cell biology · 2023Article
- M1-like, but not M0- or M2-like, macrophages, reduce RSV infection of primary bronchial epithelial cells in a media-dependent fashion.PloS one · 2022Article
- Neonatal microbiota-epithelial interactions that impact infection.Frontiers in microbiology · 2022Review
- Mulberroside A from Cortex Mori Enhanced Gut Integrity in Diabetes.Evidence-based complementary and alternative medicine : eCAM · 2021Article
- Membrane-anchored serine proteases as regulators of epithelial function.Biochemical Society transactions · 2020Review
- The Immature Gut Barrier and Its Importance in Establishing Immunity in Newborn Mammals.Frontiers in immunology · 2020Review
- Multiscale dynamics of tight junction remodeling.Journal of cell science · 2019Review
- Intestinal epithelial tight junction barrier regulation by autophagy-related protein ATG6/beclin 1.American journal of physiology. Cell physiology · 2019Article
- Occludin knockdown is not sufficient to induce transepithelial macromolecule passage.Tissue barriers · 2019Article
- Article
- Deletion of Nrf2 enhances susceptibility to eosinophilic sinonasal inflammation in a murine model of rhinosinusitis.International forum of allergy & rhinology · 2019Article
- Glucose homeostasis dependency on acini-islet-acinar (AIA) axis communication: a new possible pathophysiological hypothesis regarding diabetes mellitus.Nutrition & diabetes · 2018Review
- Cellular Tight Junctions Prevent EffectiveFrontiers in cellular and infection microbiology · 2018Article
- Qingchang Wenzhong Decoction Attenuates DSS-Induced Colitis in Rats by Reducing Inflammation and Improving Intestinal Barrier Function via Upregulating the MSP/RON Signalling Pathway.Evidence-based complementary and alternative medicine : eCAM · 2017Article
- Signaling pathways induced by serine proteases to increase intestinal epithelial barrier function.PloS one · 2017Article
Corrections and comments
- Erratum issuedCorrigendum.2016
Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
Barrier dysfunction is a characteristic of the inflammatory bowel diseases (IBD), Crohn's disease and ulcerative colitis. Understanding how the tight junction is modified to maintain barrier function may provide avenues for treatment of IBD. We have previously shown that the apical addition of serine proteases to intestinal epithelial cell lines causes a rapid and sustained increase in transepithelial electrical resistance (TER), but the mechanisms are unknown. We hypothesized that serine proteases increase barrier function through trafficking and insertion of tight junction proteins into the membrane, and this could enhance recovery of a disrupted monolayer after calcium switch or cytokine treatment. In the canine epithelial cell line, SCBN, we showed that matriptase, an endogenous serine protease, could potently increase TER. Using detergent solubility-based cell fractionation, we found that neither trypsin nor matriptase treatment changed levels of tight junction proteins at the membrane. In a fast calcium switch assay, serine proteases did not enhance the rate of recovery of the junction. In addition, serine proteases could not reverse barrier disruption induced by IFNγ and TNFα. We knocked down occludin in our cells using siRNA and found this prevented the serine protease-induced increase in TER. Using fluorescence recovery after photobleaching (FRAP), we found serine proteases induce a greater mobile fraction of occludin in the membrane. These data suggest that a functional tight junction is needed for serine proteases to have an effect on TER, and that occludin is a crucial tight junction protein in this mechanism.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.