Evidence map›Paper›PMID 27492182›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2016

Assessment of the Contractile Properties of Permeabilized Skeletal Muscle Fibers.

Dennis R Claflin, Stuart M Roche, Jonathan P Gumucio, Christopher L Mendias, Susan V Brooks

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Single Muscle Fibre Contractility Testing in Rats to Quantify Ischaemic Muscle Damage During Reperfusion Injury.European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery · 2019
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Dennis R ClaflinDepartment of Surgery, Section of Plastic Surgery, University of Michigan Medical School, Ann Arbor, MI, USA.
Stuart M RocheDepartment of Orthopaedic Surgery, University of Michigan Medical School, Ann Arbor, MI, USA.
Jonathan P GumucioDepartment of Orthopaedic Surgery, University of Michigan Medical School, Ann Arbor, MI, USA.
Christopher L MendiasDepartment of Orthopaedic Surgery, University of Michigan Medical School, Ann Arbor, MI, USA.
Susan V BrooksDepartment of Biomedical Engineering, University of Michigan College of Engineering, Ann Arbor, MI, USA. svbrooks@umich.edu.

Funding

PROJECT 3: Neuromuscular redox homeostasis in mice lacking SOD1 and aging wild type mice subcontract at University of LiverpoolP01AG051442 · NIA · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI JACKSON, MALCOLM JOSEPH · 2016 to 2020
$8.9M
Sex Differences in Tendon Growth and RemodelingR01AR063649 · NIAMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI RODEO, SCOTT A · 2013 to 2022
$3.6M
Defining the relative roles of pre- and post-synaptic events in the initiation and progression of sarcopeniaR01AG050676 · NIA · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI BROOKS, SUSAN V, VAN REMMEN, HOLLY · 2016 to 2020
$3.3M
Fatty Macrophage Regulation of Atrophy and Inflammation in Rotator Cuff TearsF31AR065931 · NIAMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI GUMUCIO, JONATHAN PAUL · 2014 to 2016
$103k
NIAMS NIH HHS F31 AR065931NIAMS NIH HHS R01 AR063649NIA NIH HHS P01 AG051442NIA NIH HHS R01 AG050676
6 · The paper itself

Abstract

Permeabilized individual skeletal muscle fibers offer the opportunity to evaluate contractile behavior in a system that is greatly simplified, yet physiologically relevant. Here we describe the steps required to prepare, permeabilize and preserve small samples of skeletal muscle. We then detail the procedures used to isolate individual fiber segments and attach them to an experimental apparatus for the purpose of controlling activation and measuring force generation. We also describe our technique for estimating the cross-sectional area of fiber segments. The area measurement is necessary for normalizing the absolute force to obtain specific force, a measure of the intrinsic force-generating capability of the contractile system.

Indexed as

AnimalsMuscle ContractionMuscle Fibers, SkeletalMuscle, SkeletalMuscle StrengthContractilityCross-sectional areaIsometric forceMuscle physiologyPermeabilized muscle fiberSingle muscle fiberSkeletal muscleSpecific force

Identifiers

PMID27492182
PMCPMC11423931

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.