ArticleMethods in molecular biology (Clifton, N.J.)2016
Skeletal Muscle Tissue Clearing for LacZ and Fluorescent Reporters, and Immunofluorescence Staining.
Article in Methods in molecular biology (Clifton, N.J.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed.
- Protocol for the three-dimensional analysis of rodent skeletal muscle.STAR protocols · 2025Article
- From morphology to single-cell molecules: high-resolution 3D histology in biomedicine.Molecular cancer · 2025Review
- Three-Dimensional Analysis of Skeletal Muscle Stem Cell Niche Following Tissue Clearing.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Imaging analysis for muscle stem cells and regeneration.Frontiers in cell and developmental biology · 2024Review
- Review
- Tissue Clearing and Confocal Microscopic Imaging for Skeletal Muscle.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Three-Dimensional Imaging Analysis for Skeletal Muscle.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Visualization of the Skeletal Muscle Stem Cell Niche in Fiber Bundles.Current protocols · 2021Article
- Translational research on Myology and Mobility Medicine: 2021 semi-virtual PDM3 from Thermae of Euganean Hills, May 26 - 29, 2021.European journal of translational myology · 2021Article
- Enhancing Interrogation of Skeletal Muscle Samples for Informative Quantitative Data.Journal of neuromuscular diseases · 2021Review
- Biomedical Applications of Tissue Clearing and Three-Dimensional Imaging in Health and Disease.iScience · 2020Review
- Muscle Satellite Cell Cross-Talk with a Vascular Niche Maintains Quiescence via VEGF and Notch Signaling.Cell stem cell · 2018Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Skeletal muscle is a highly ordered yet complex tissue containing several cell types that interact with each other in order to maintain structure and homeostasis. It is also a highly regenerative tissue that responds to damage in a highly intricate but stereotypic manner, with distinct spatial and temporal kinetics. Proper examination of this process requires one to look at the three-dimensional orientation of the cellular and subcellular components, which can be accomplished through tissue clearing. While there has been a recent surge of protocols to study biology in whole tissue, it has primarily focused on the nervous system. This chapter describes the workflow for whole mount analysis of murine skeletal muscle for LacZ reporters, fluorescent reporters and immunofluorescence staining. Using this technique, we are able to visualize LacZ reporters more effectively in deep tissue samples, and to perform fluorescent imaging with a depth greater than 1700 μm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.