ArticleScientific reports2016
Two-photon excited fluorescence of intrinsic fluorophores enables label-free assessment of adipose tissue function.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 36 citations in OpenAlex.
- Multispectral laser-scanning pulse-sampling fluorescence lifetime system for large-scale tissue imaging.Optics letters · 2025Article
- Label-Free Optical Metabolic Imaging in Cells and Tissues.Annual review of biomedical engineering · 2023Review
- Label-free, multi-parametric assessments of cell metabolism and matrix remodeling within human and early-stage murine osteoarthritic articular cartilage.Communications biology · 2023Article
- Light distribution in fat cell layers at physiological temperatures.Scientific reports · 2023Article
- Label-free optical metabolic imaging of adipose tissues for prediabetes diagnosis.Theranostics · 2023Article
- Coordinated metabolic transitions and gene expression by NAD+ during adipogenesis.The Journal of cell biology · 2022Article
- Riboflavin depletion promotes longevity and metabolic hormesis in Caenorhabditis elegans.Aging cell · 2022Article
- Nicotinamide effects on the metabolism of human fibroblasts and keratinocytes assessed by quantitative, label-free fluorescence imaging.Biomedical optics express · 2021Article
- Tissue Imaging and Quantification Relying on Endogenous Contrast.Advances in experimental medicine and biology · 2021Review
- Cancer cells with defective oxidative phosphorylation require endoplasmic reticulum-to-mitochondria CaScience signaling · 2020Article
- Nonlinear Optical Microscopy: From Fundamentals to Applications in Live Bioimaging.Frontiers in bioengineering and biotechnology · 2020Review
- Optical changes in THP-1 macrophage metabolism in response to pro- and anti-inflammatory stimuli reported by label-free two-photon imaging.Journal of biomedical optics · 2020Article
- 3D extracellular matrix microenvironment in bioengineered tissue models of primary pediatric and adult brain tumors.Nature communications · 2019Article
- Simultaneous label-free autofluorescence-multiharmonic microscopy and beyond.APL photonics · 2019Article
- Slide-free virtual histochemistry (Part I): development via nonlinear optics.Biomedical optics express · 2018Article
- Serum and Hepatic Autofluorescence as a Real-Time Diagnostic Tool for Early Cholestasis Assessment.International journal of molecular sciences · 2018Article
- Enhanced quantification of metabolic activity for individual adipocytes by label-free FLIM.Scientific reports · 2018Article
- Mapping metabolic changes by noninvasive, multiparametric, high-resolution imaging using endogenous contrast.Science advances · 2018Article
- Non-Euclidean phasor analysis for quantification of oxidative stress in ex vivo human skin exposed to sun filters using fluorescence lifetime imaging microscopy.Journal of biomedical optics · 2017Article
- Label free monitoring of megakaryocytic development and proplatelet formation in vitro.Biomedical optics express · 2017Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Current methods for evaluating adipose tissue function are destructive or have low spatial resolution. These limit our ability to assess dynamic changes and heterogeneous responses that occur in healthy or diseased subjects, or during treatment. Here, we demonstrate that intrinsic two-photon excited fluorescence enables functional imaging of adipocyte metabolism with subcellular resolution. Steady-state and time-resolved fluorescence from intracellular metabolic co-factors and lipid droplets can distinguish the functional states of excised white, brown, and cold-induced beige fat. Similar optical changes are identified when white and brown fat are assessed in vivo. Therefore, these studies establish the potential of non-invasive, high resolution, endogenous contrast, two-photon imaging to identify distinct adipose tissue types, monitor their functional state, and characterize heterogeneity of induced responses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.