ArticleMolecules (Basel, Switzerland)2016
Potential Application of p-Coumaric Acid on Differentiation of C2C12 Skeletal Muscle and 3T3-L1 Preadipocytes-An in Vitro and in Silico Approach.
Article in Molecules (Basel, Switzerland), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.
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- Anti-Obesogenic Effects of Culinary Herbs Through Modulation of Inflammation and Metabolic Pathways.Nutrients · 2026Review
- Predicting the bioactive compounds ofHeliyon · 2024Article
- Phytochemical characterization ofFrontiers in chemistry · 2023Article
- Bioactive Components in Whole Grains for the Regulation of Skeletal Muscle Function.Foods (Basel, Switzerland) · 2022Review
- Sinapic acid prevents adipogenesis by regulating transcription factors and exerts an anti-ROS effect by modifying the intracellular anti-oxidant system in 3T3-L1 adipocytes.Iranian journal of basic medical sciences · 2022Article
- Effect of Tomato Peel Extract Grown under Drought Stress Condition in a Sarcopenia Model.Molecules (Basel, Switzerland) · 2022Article
- Role of Exosomal miR-223 in Chronic Skeletal Muscle Inflammation.Orthopaedic surgery · 2022Review
- Bioinformatic analysis of the gene expression profile in muscle atrophy after spinal cord injury.Scientific reports · 2021Article
- Influence of myrcene on inflammation, matrix accumulation in the kidney tissues of streptozotocin-induced diabetic rat.Saudi journal of biological sciences · 2021Article
- Polyphenols and Their Effects on Muscle Atrophy and Muscle Health.Molecules (Basel, Switzerland) · 2021Review
- Sinapic Acid Promotes Browning of 3T3-L1 Adipocytes via p38 MAPK/CREB Pathway.BioMed research international · 2020Article
- Influence of olive oil and its components on mesenchymal stem cell biology.World journal of stem cells · 2019Review
- High-Fructose Diet-Induced Metabolic Disorders Were Counteracted by the Intake of Fruit and Leaves of Sweet Cherry in Wistar Rats.Nutrients · 2019Article
- Modulation of osteogenic and myogenic differentiation by a phytoestrogen formononetin via p38MAPK-dependent JAK-STAT and Smad-1/5/8 signaling pathways in mouse myogenic progenitor cells.Scientific reports · 2019Article
- Natural Products and Obesity: A Focus on the Regulation of Mitotic Clonal Expansion during Adipogenesis.Molecules (Basel, Switzerland) · 2019Review
- Article
- The Modulatory Properties of Li-Ru-Kang Treatment on Hyperplasia of Mammary Glands Using an Integrated Approach.Frontiers in pharmacology · 2018Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coumaric acid (CA) is a phenolic acid of the hydroxycinnamic acid family, and it has many biological functions such as anti-oxidant, anti-inflammatory, antidiabetic, anti-ulcer, anti-platelet, anti-cancer activities, etc. In the present study, we planned to analyse the potential molecular function of CA on skeletal muscle and preadipocytes differentiation using PCR and Western blot techniques. First, we analysed the impact of CA on C2C12 skeletal muscle differentiation. It revealed that CA treatment inhibited horse serum-induced skeletal muscle differentiation as evidenced by the decreased expression of early myogenic differentiation markers such as Myogenin and myoD via the AMP activated protein kinase- alpha AMPK-α mediated pathway. Furthermore, the level of lipid accumulation and changes in genes and protein expressions that are associated with lipogenesis and lipolysis were analyzed in 3T3-L1 cells. The Oil Red O staining evidenced that CA treatment inhibited lipid accumulation at the concentration of 0.1 and 0.2 mM. Furthermore, coumaric acid treatment decreased the expression of main transcriptional factors such as CCAAT/enhancer binding protein-alpha (C/EBP-α) and peroxisome proliferator-activated receptor gamma-2 (PPAR-γ2). Subsequently, CA treatment decreased the expression of sterol regulatory element binding protein-1 (SREBP-1), fatty acid synthase (FAS), acetyl CoA carboxylase (ACC) and adiponectin. Finally, we identified conformational changes induced by CA in PPAR-γ2 using computational biology tools. It revealed that CA might downregulate the PPAR-γ2 expression by directly binding with amino acids of PPAR-γ2 by hydrogen at 3.26 distance and hydrophobic interactions at 3.90 contact distances. These data indicated that CA suppressed skeletal muscle and preadipocytes differentiation through downregulation of the main transcriptional factors and their downstream targets.
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