ArticlePloS one2016
Gateway Vectors for Simultaneous Detection of Multiple Protein-Protein Interactions in Plant Cells Using Bimolecular Fluorescence Complementation.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed.
- INDETERMINATE DOMAIN-DELLA protein interactions orchestrate gibberellin-mediated cell elongation in wheat and barley.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Nucleo-cytoplasmic distribution of SAP18 reveals its dual function in splicing regulation and heat-stress response in Arabidopsis.Plant communications · 2025Article
- BIL7 enhances plant growth by regulating the transcription factor BIL1/BZR1 during brassinosteroid signaling.The Plant journal : for cell and molecular biology · 2025Article
- Powdery mildew effectors AVRMolecular plant pathology · 2024Article
- SreC-dependent adaption to host iron environments regulates the transition of trophic stages and developmental processes of Curvularia lunata.Molecular plant pathology · 2024Article
- Evolutionarily conserved protein motifs drive interactions between the plant nucleoskeleton and nuclear pores.The Plant cell · 2023Article
- Phosphorylation and ubiquitination of OsWRKY31 are integral to OsMKK10-2-mediated defense responses in rice.The Plant cell · 2023Article
- The bZIP transcription factor AREB3 mediates FT signalling and floral transition at the Arabidopsis shoot apical meristem.PLoS genetics · 2023Article
- Barley endosomal MONENSIN SENSITIVITY1 is a target of the powdery mildew effector CSEP0162 and plays a role in plant immunity.Journal of experimental botany · 2023Article
- Application of a novel strong promoter from Chinese fir (Frontiers in plant science · 2023Article
- The grapevine CAX-interacting protein VvCXIP4 is exported from the nucleus to activate the tonoplast CaPlanta · 2020Article
- Comprehensive Identification of PTI Suppressors in Type III Effector Repertoire Reveals thatInternational journal of molecular sciences · 2019Article
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7 authors.
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Abstract
Bimolecular fluorescence complementation (BiFC) is widely used to detect protein-protein interactions, because it is technically simple, convenient, and can be adapted for use with conventional fluorescence microscopy. We previously constructed enhanced yellow fluorescent protein (EYFP)-based Gateway cloning technology-compatible vectors. In the current study, we generated new Gateway cloning technology-compatible vectors to detect BiFC-based multiple protein-protein interactions using N- and C-terminal fragments of enhanced cyan fluorescent protein (ECFP), enhanced green fluorescent protein (EGFP), and monomeric red fluorescent protein (mRFP1). Using a combination of N- and C-terminal fragments from ECFP, EGFP and EYFP, we observed a shift in the emission wavelength, enabling the simultaneous detection of multiple protein-protein interactions. Moreover, we developed these vectors as binary vectors for use in Agrobacterium infiltration and for the generate transgenic plants. We verified that the binary vectors functioned well in tobacco cells. The results demonstrate that the BiFC vectors facilitate the design of various constructions and are convenient for the detection of multiple protein-protein interactions simultaneously in plant cells.
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