ArticleNew microbes and new infections2016
Real-time quantitative PCR assay with Taqman(®) probe for rapid detection of MCR-1 plasmid-mediated colistin resistance.
Article in New microbes and new infections, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers, 1 of them a synthesis that pooled it.
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Who cites it
43 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Mcr colistin resistance gene: a systematic review of current diagnostics and detection methods.MicrobiologyOpen · 2019Pooled it
- Colistin Resistance in Gram-Negative Bacteria: Mechanisms, Transmission, and Novel Intervention Strategies.Microorganisms · 2026Review
- The colistin resistance pandemic: A dual threat from plasmid-encoded mobilised colistin resistance genes and chromosomal mutations.African journal of laboratory medicine · 2026Review
- The Public Health Risks of Colistin Resistance in Dogs and Cats: A One Health Perspective Review.Antibiotics (Basel, Switzerland) · 2025Review
- Colistin- and cefotaxime-resistant Shiga toxin-producing Escherichia coli (STEC) in buffalo meat.Scientific reports · 2025Article
- The Regulation of the Albomycin and Desferrioxamine E Biosynthesis inMolecules (Basel, Switzerland) · 2025Article
- Detection assay of polymyxin resistance codingFrontiers in cellular and infection microbiology · 2025Article
- Whole genome analysis of multidrug-resistantMicrobiology spectrum · 2024Article
- Development and evaluation of rapid and accurate one-tube RPA-CRISPR-Cas12b-based detection of mcr-1 and tet(X4).Applied microbiology and biotechnology · 2024Article
- A Review on Colistin Resistance: An Antibiotic of Last Resort.Microorganisms · 2024Review
- Challenges in the Detection of Polymyxin Resistance: From Today to the Future.Microorganisms · 2024Review
- Phenotypic and genotypic characterization of extended spectrum beta-lactamase producingOpen veterinary journal · 2024Article
- First report of detection ofIranian journal of veterinary research · 2024Article
- A Review of Resistance to Polymyxins and Evolving Mobile Colistin Resistance Gene (Antibiotics (Basel, Switzerland) · 2023Review
- Mobile Colistin Resistance (Antibiotics (Basel, Switzerland) · 2023Review
- Dissemination of Carbapenemases and MCR-1 Producing Gram-Negative Bacteria in Aquatic Environments in Batna, Algeria.Antibiotics (Basel, Switzerland) · 2022Article
- Occurrence of Extended Spectrum Cephalosporin-, Carbapenem- and Colistin-Resistant Gram-Negative Bacteria in Fresh Vegetables, an Increasing Human Health Concern in Algeria.Antibiotics (Basel, Switzerland) · 2022Article
- Phenotypic and Genotypic Screening of Colistin Resistance Associated with Emerging PathogenicVeterinary sciences · 2022Article
- Detection ofMicroorganisms · 2022Article
- Screening of Colistin-Resistant Bacteria in Domestic Pets from France.Animals : an open access journal from MDPI · 2022Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we report the development of two rapid real-time quantitative PCR assays with TaqMan(®) probes to detect the MCR-1 plasmid-mediated colistin resistance gene from bacterial isolates and faecal samples from chickens. Specificity and sensitivity of the assay were 100% on bacterial isolates including 18 colistin-resistant isolates carrying the mcr-1 gene (six Klebsiella pneumoniae and 12 Escherichia coli) with a calibration curve that was linear from 10(1) to 10(8) DNA copies. Five out of 833 faecal samples from chickens from Algeria were positive, from which three E. coli strains were isolated and confirmed to harbour the mcr-1 gene by standard PCR and sequencing.
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Registered trials
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