ArticleJournal of neurophysiology2016
High-fidelity optical excitation of cortico-cortical projections at physiological frequencies.
Article in Journal of neurophysiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 37 citations in OpenAlex.
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- Deep brain stimulation alleviates Parkinsonian motor deficits through desynchronizing GABA release in mice.Nature communications · 2025Article
- Synaptic Engram.Advances in neurobiology · 2024Review
- Impact of volume and expression time in an AAV-delivered channelrhodopsin.Molecular brain · 2023Article
- Optogenetic stimulation of anterior insular cortex neurons in male rats reveals causal mechanisms underlying suppression of the default mode network by the salience network.Nature communications · 2023Article
- Simultaneous Electrophysiology and Optogenetic Perturbation of the Same Neurons in Chronically Implanted Animals using μLED Silicon Probes.bioRxiv : the preprint server for biology · 2023Article
- Short-Term Facilitation of Long-Range Corticocortical Synapses Revealed by Selective Optical Stimulation.Cerebral cortex (New York, N.Y. : 1991) · 2022Article
- High-performance microbial opsins for spatially and temporally precise perturbations of large neuronal networks.Neuron · 2022Article
- The Effect of Serotonin Receptor 5-HT1B on Lateral Inhibition between Spiny Projection Neurons in the Mouse Striatum.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Prefrontal Cortex-Driven Dopamine Signals in the Striatum Show Unique Spatial and Pharmacological Properties.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Context-dependent and dynamic functional influence of corticothalamic pathways to first- and higher-order visual thalamus.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- The Optogenetic Revolution in Cerebellar Investigations.International journal of molecular sciences · 2020Review
- Light Up the Brain: The Application of Optogenetics in Cell-Type Specific Dissection of Mouse Brain Circuits.Frontiers in neural circuits · 2020Review
- Article
- Manipulating fear associations via optogenetic modulation of amygdala inputs to prefrontal cortex.Nature neuroscience · 2017Article
- Activation of Distinct Channelrhodopsin Variants Engages Different Patterns of Network Activity.eNeuroArticle
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Optogenetic activation of axons is a powerful approach for determining the synaptic properties and impact of long-range projections both in vivo and in vitro. However, because of the difficulty of measuring activity in axons, our knowledge of the reliability of optogenetic axonal stimulation has relied on data from somatic recordings. Yet, there are many reasons why activation of axons may not be comparable to cell bodies. Thus we have developed an approach to more directly assess the fidelity of optogenetic activation of axonal projections. We expressed opsins (ChR2, Chronos, or oChIEF) in the mouse primary visual cortex (V1) and recorded extracellular, pharmacologically isolated presynaptic action potentials in response to axonal activation in the higher visual areas. Repetitive stimulation of axons with ChR2 resulted in a 70% reduction in the fiber volley amplitude and a 60% increase in the latency at all frequencies tested (10-40 Hz). Thus ChR2 cannot reliably recruit axons during repetitive stimulation, even at frequencies that are reliable for somatic stimulation, likely due to pronounced channel inactivation at the high light powers required to evoke action potentials. By comparison, oChIEF and Chronos evoked photocurrents that inactivated minimally and could produce reliable axon stimulation at frequencies up to 60 Hz. Our approach provides a more direct and accurate evaluation of the efficacy of new optogenetic tools and has identified Chronos and oChIEF as viable tools to interrogate the synaptic and circuit function of long-range projections.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.