ArticleMolecular biology of the cell2016
Microtubule aging probed by microfluidics-assisted tubulin washout.
Article in Molecular biology of the cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Development of a Microfluidics-Based Approach for Investigating Microtubule Polymer Mechanics.Journal of visualized experiments : JoVE · 2025Article
- EB3-informed dynamics of the microtubule stabilizing cap during stalled growth.Biophysical journal · 2025Article
- Modulation of microtubule dynamics by monovalent ions.PNAS nexus · 2025Article
- Interface-acting nucleotide controls polymerization dynamics at microtubule plus- and minus-ends.eLife · 2024Article
- Reliable and robust control of nucleus centering is contingent on nonequilibrium force patterns.iScience · 2023Article
- CSPP1 stabilizes growing microtubule ends and damaged lattices from the luminal side.The Journal of cell biology · 2023Article
- Dynamic microtubules slow down during their shrinkage phase.Biophysical journal · 2023Article
- Theory of tip structure-dependent microtubule catastrophes and damage-induced microtubule rescues.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Quantification of microtubule stutters: dynamic instability behaviors that are strongly associated with catastrophe.Molecular biology of the cell · 2022Article
- Thermal hysteresis in microtubule assembly/disassembly dynamics: The aging-induced degradation of tubulin dimers.Biochemistry and biophysics reports · 2022Article
- Atomic force microscopy reveals distinct protofilament-scale structural dynamics in depolymerizing microtubule arrays.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Lattice defects induced by microtubule-stabilizing agents exert a long-range effect on microtubule growth by promoting catastrophes.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
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- Non-enzymatic Activity of the α-Tubulin Acetyltransferase αTAT Limits Synaptic Bouton Growth in Neurons.Current biology : CB · 2020Article
- Multiscale Computational Modeling of Tubulin-Tubulin Lateral Interaction.Biophysical journal · 2019Article
- Long-range, through-lattice coupling improves predictions of microtubule catastrophe.Molecular biology of the cell · 2019Article
- Direct observation of individual tubulin dimers binding to growing microtubules.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- A unified model for microtubule rescue.Molecular biology of the cell · 2019Article
- Microtubule Plus End Dynamics - Do We Know How Microtubules Grow?: Cells boost microtubule growth by promoting distinct structural transitions at growing microtubule ends.BioEssays : news and reviews in molecular, cellular and developmental biology · 2019Article
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3 authors.
Funding
Abstract
Microtubules switch stochastically between phases of growth and shrinkage. The molecular mechanism responsible for the end of a growth phase, an event called catastrophe, is still not understood. The probability for a catastrophe to occur increases with microtubule age, putting constraints on the possible molecular mechanism of catastrophe induction. Here we used microfluidics-assisted fast tubulin washout experiments to induce microtubule depolymerization in a controlled manner at different times after the start of growth. We found that aging can also be observed in this assay, providing valuable new constraints against which theoretical models of catastrophe induction can be tested. We found that the data can be quantitatively well explained by a simple kinetic threshold model that assumes an age-dependent broadening of the protective cap at the microtubule end as a result of an evolving tapered end structure; this leads to a decrease of the cap density and its stability. This analysis suggests an intuitive picture of the role of morphological changes of the protective cap for the age dependence of microtubule stability.
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Registered trials
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